Pettersson Ann, Nilsson Linn, Nylund Gunnar, Khorram-Manesh Amir, Nordgren Svante, Delbro Dick S
School of Pure and Applied Natural Sciences, University of Kalmar, SE-39182 Kalmar, Sweden.
Eur J Pharmacol. 2009 May 1;609(1-3):27-33. doi: 10.1016/j.ejphar.2009.03.002. Epub 2009 Mar 11.
We used immunochemistry to demonstrate expression of acetylcholine's nicotinic alpha7-receptor subtype in human colon cancer cell line HT-29. Moreover, RT-PCR and immunochemistry showed that choline acetyltransferase and acetylcholine esterase, the enzymes responsible for acetylcholine synthesis and degradation, respectively, localise in HT-29 cells. Bromoacetylcholine bromide, an inhibitor of choline acetyltransferase, significantly attenuated basal cell growth. Our findings suggest that acetylcholine might serve as an autocrine/paracrine-or speculatively, even intracrine-signalling molecule in cell line HT-29, thus contributing to carcinogenesis/cancer progression.
我们运用免疫化学方法来证明人结肠癌细胞系HT - 29中乙酰胆碱烟碱α7受体亚型的表达。此外，逆转录聚合酶链反应（RT - PCR）和免疫化学显示，分别负责乙酰胆碱合成和降解的胆碱乙酰转移酶和乙酰胆碱酯酶定位于HT - 29细胞中。胆碱乙酰转移酶抑制剂溴化溴乙酰胆碱显著减弱了基础细胞生长。我们的研究结果表明，乙酰胆碱可能作为细胞系HT - 29中的自分泌/旁分泌信号分子，或者推测甚至作为胞内分泌信号分子，从而促进致癌作用/癌症进展。
