Adams Stuart J, Fussell Richard J, Dickinson Mike, Wilkins Selwyn, Sharman Matthew
Food Science Group, Central Science Laboratory, Sand Hutton, York YO41 1LZ, United Kingdom.
Anal Chim Acta. 2009 Apr 1;637(1-2):315-20. doi: 10.1016/j.aca.2008.09.013. Epub 2008 Sep 11.
Bee colonies were treated with 1.2g lincomycin hydrochloride per hive (single treatment in sucrose solution) and samples of honey were then collected at intervals over a 41-week period. The samples were analysed for lincomycin using Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS). The highest mean concentration of lincomycin (pooled analytical results for brood and super honey) was 24 microugg(-1) 3 days after treatment, a mean of 3.5 microgg(-1) after 129 days. The shook swarm procedure was investigated and resulted in a lincomycin concentration of 34 microgg(-1) in honey (pooled results for brood and super honey) 3 days after treatment, declining to 0.38 microgg(-1) 129 days after treatment. Lincomycin was persistent in the hive and detected in all over winter (290 days after dosing) samples of honey collected from both non-shook swarmed and shook swarmed colonies. The results overall indicate that lincomycin parent is a suitable marker compound to detect lincomycin misuse in apiculture.
每个蜂群用每箱1.2克盐酸林可霉素（在蔗糖溶液中单次处理）进行处理，然后在41周的时间内定期采集蜂蜜样本。使用液相色谱 - 质谱联用/质谱（LC-MS/MS）对样本中的林可霉素进行分析。处理后3天，林可霉素的最高平均浓度（育虫蜂蜜和继箱蜂蜜的合并分析结果）为24微克/克，129天后平均为3.5微克/克。对摇蜜群程序进行了研究，处理后3天蜂蜜中林可霉素浓度（育虫蜂蜜和继箱蜂蜜的合并结果）为34微克/克，处理后129天降至0.38微克/克。林可霉素在蜂巢中具有持久性，在从非摇蜜群和摇蜜群蜂群采集的所有越冬（给药后290天）蜂蜜样本中均有检测到。总体结果表明，林可霉素母体是检测养蜂业中林可霉素滥用情况的合适标记化合物。
