Thompson Helen M, Waite Ruth J, Wilkins Selwyn, Brown Michael A, Bigwood Tim, Shaw Marvin, Ridgway Christopher, Sharman Matthew
National Bee Unit, Central Science Laboratory, Sand Hutton, York, UK.
Food Addit Contam. 2005 Jun;22(6):573-8. doi: 10.1080/02652030500089986.
This study aimed to assess oxytetracycline (OTC) residue levels in honey up to 12 weeks after treatment of honeybee colonies with two methods of application (in liquid sucrose and in powdered icing sugar). Significantly greater mortality was seen in the all stages of brood development for the treated colonies when compared with the controls. Samples of honey were extracted up to 12 weeks after treatment and analysed by HPLC following metal chelation with a limit of quantitation of 0.05 mg/kg. These data showed that the current method of application of Terramycin in liquid form results in very high residue levels in honey with residues of 3.7 mg/kg eight weeks after application. Further work is required to determine whether the levels can be further reduced by changes in the method of application whilst ensuring efficacy and minimizing the effects on brood.
本研究旨在评估采用两种施用方法（液态蔗糖和糖粉）处理蜂群后长达12周内蜂蜜中土霉素（OTC）的残留水平。与对照组相比，处理过的蜂群在所有幼虫发育阶段的死亡率均显著更高。处理后长达12周采集蜂蜜样本，经金属螯合后采用高效液相色谱法（HPLC）分析，定量限为0.05 mg/kg。这些数据表明，目前以液体形式施用土霉素的方法导致蜂蜜中残留水平非常高，施用八周后残留量为3.7 mg/kg。需要进一步开展工作，以确定能否通过改变施用方法进一步降低残留水平，同时确保药效并将对幼虫的影响降至最低。
