Dickinson Michelle A, Harnett Erica L, Venditti Carolina C, Smith Graeme N
Department of Anatomy and Cell Biology, Queen's University, Kingston, ON, Canada.
Am J Obstet Gynecol. 2009 May;200(5):534.e1-6. doi: 10.1016/j.ajog.2008.12.034. Epub 2009 Mar 16.
The aim of this study was to characterize the pregnant guinea pig cytokine time course following a maternal inflammatory insult.
Pregnant guinea pigs (n = 34) were injected intraperitoneally with 100 microg/kg lipopolysaccharide (LPS) at 70% gestation and euthanized at 24 hours, 48 hours, or 5 days. Control animals were euthanized at 70% gestation without LPS exposure. Interleukin (IL)-6, IL-1beta, and tumor necrosis factor-alpha (TNF-alpha) were quantified in the maternal serum and amniotic fluid by an enzyme-linked immunosorbent assay.
IL-6 and IL-1beta concentrations were elevated in the maternal serum at 24 hours and returned to control levels by 5 days. In the amniotic fluid, IL-6 peaked at 48 hours and IL-1beta at 24 hours. TNF-alpha levels were not significantly increased.
Maternal intraperitoneal LPS injection produces transient increases in cytokine concentrations in the maternal serum and amniotic fluid within 5 days, further implicating the cytokines as mediators of fetal white matter damage.
本研究旨在描述孕豚鼠在母体发生炎性损伤后的细胞因子时间进程。
34只孕豚鼠在妊娠70%时腹腔注射100微克/千克脂多糖（LPS），并在24小时、48小时或5天时实施安乐死。对照动物在妊娠70%时未接触LPS即实施安乐死。通过酶联免疫吸附测定法对母体血清和羊水中的白细胞介素（IL）-6、IL-1β和肿瘤坏死因子-α（TNF-α）进行定量分析。
母体血清中的IL-6和IL-1β浓度在24小时时升高，并在5天时恢复至对照水平。羊水中，IL-6在48小时时达到峰值，IL-1β在24小时时达到峰值。TNF-α水平未显著升高。
母体腹腔注射LPS可在5天内使母体血清和羊水中的细胞因子浓度产生短暂升高，进一步表明细胞因子是胎儿白质损伤的介质。
