Institute of Molecular Pathogenesis in the Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Naumburger Str. 96a, 07743 Jena, Germany.
Vet J. 2010 May;184(2):212-8. doi: 10.1016/j.tvjl.2009.02.005. Epub 2009 Mar 14.
This study characterises the acid-base abnormalities in pigs experimentally infected with Chlamydia suis (Henderson-Hasselbalch equation and Constable's simplified strong ion equation). Eight pigs were challenged with the respiratory pathogen C. suis and four pigs served as non-infected controls. Pigs were monitored from 7 days before challenge to 8 days post-inoculation. Clinical examination was performed twice daily and venous blood samples were collected every two days. Blood-gas analysis, haemoxymetry, serum biochemical analysis and electrophoresis were performed in order to characterise the acid-base derangement. Aerosol challenge with C. suis resulted in severe acid-base disturbance characterised by acute respiratory acidosis and strong ion (metabolic) acidosis secondary to anaerobic metabolism and hyper L-lactataemia. Maximal changes were seen at day 3 post-inoculation when severe clinical signs of respiratory dysfunction were evident. The results of the study provide new information regarding the pathophysiology of respiratory infection caused by C. suis and the applicability and diagnostic utility of different approaches for assessing acid-base status in pigs.
本研究描述了实验感染猪肺炎支原体（亨德森-哈塞尔巴尔赫方程和康斯特布尔简化强离子方程）后的酸碱失衡。将 8 头猪用呼吸道病原体猪肺炎支原体进行攻毒，4 头猪作为非感染对照。从攻毒前 7 天开始监测猪，直到攻毒后 8 天。每天进行两次临床检查，并每隔两天采集静脉血样。进行血气分析、血氧测定、血清生化分析和电泳，以描述酸碱失调情况。用猪肺炎支原体进行气溶胶攻毒会导致严重的酸碱失衡，表现为急性呼吸性酸中毒和强离子（代谢性）酸中毒，继发于无氧代谢和高 L-乳酸血症。在接种后第 3 天，当明显出现严重的呼吸功能障碍临床症状时，出现了最大的变化。该研究的结果提供了有关猪肺炎支原体引起的呼吸道感染的病理生理学以及评估猪酸碱状态的不同方法的适用性和诊断效用的新信息。
