Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, SI-1000 Ljubljana, Slovenia.
Stud Mycol. 2008;61:51-9. doi: 10.3114/sim.2008.61.04.
Multiple tolerance to stressful environmental conditions of the black, yeast-like fungus Aureobasidium pullulans is achieved through different adaptations, among which there is the restructuring of the lipid composition of their membranes. Here, we describe three novel genes encoding fatty-acid-modifying enzymes in A. pullulans, along with the levels of their mRNAs under different salinity conditions. High levels of Delta(9)-desaturase and Delta(12)-desaturase mRNAs were seen at high salinities, which were consistent with an increased desaturation of the fatty acids in the cell membranes. Elevated levels of elongase mRNA were also detected. Surprisingly, increases in the levels of these mRNAs were also seen following hypo-osmotic shock, while hyperosmotic shock had exactly the opposite effect, demonstrating that data that are obtained from up-shift and down-shift salinity studies should be interpreted with caution.
黑酵母样真菌出芽短梗霉能够耐受多种应激环境条件，这是通过不同的适应方式实现的，其中包括其膜脂组成的重构。在这里，我们描述了出芽短梗霉中三个编码脂肪酸修饰酶的新基因，并研究了它们在不同盐度条件下的 mRNA 水平。在高盐度下，观察到 Delta(9)-去饱和酶和 Delta(12)-去饱和酶 mRNA 的水平较高，这与细胞膜中脂肪酸的去饱和增加一致。还检测到延伸酶 mRNA 水平的升高。令人惊讶的是，在低渗冲击后也观察到这些 mRNA 水平的增加，而高渗冲击则有完全相反的效果，这表明从盐度上调和下调研究中获得的数据应该谨慎解释。
