Ramirez-Castrillon Mauricio, Benavides-León Tatiana Andrea, Arcos-Velasco Lizeth Vanessa, Pantoja-Pulido Kriss Dayana, Lopez-Parra Lizbeth Lorena, Bolaños-Rojas Ana Cristina, Osorio-Cadavid Esteban
Programa de Microbiología, Universidad Santiago de Cali, Calle 5 62-00, Cali, 760035, Valle del Cauca, Colombia.
Escuela de Microbiología, Facultad de Salud, Universidad Industrial de Santander, Carrera 32 29-31, Bucaramanga, 680002, Santander, Colombia.
World J Microbiol Biotechnol. 2025 Mar 13;41(3):105. doi: 10.1007/s11274-025-04309-7.
Oleaginous yeasts have emerged as promising microbial cell factories for lipid production, offering sustainable alternatives to traditional sources of biodiesel and nutraceuticals. In this study, the lipid accumulation potential of yeast strains isolated from two freshwater aquatic ecosystems in Cali, Colombia, was evaluated to identify novel candidates for biotechnological applications. A total of 56 strains were tested for their oleaginous nature using a gravimetric lipid assay with glucose as a carbon source. Of the assessed strains, 46.15% exceeded 20% lipid yields relative to the dry biomass. Seven strains were selected using glycerol as a carbon source, but only five yeasts were further characterized for their lipid profiles. Molecular identification revealed diverse species, including Aureobasidium sp., Papiliotrema rajashtanensis, Rhodotorula spp., and Clavispora lusitaniae. The selected strains demonstrated unique lipid profiles, with high proportions of monounsaturated and polyunsaturated fatty acids, such as oleic acid (C18:1) and linoleic acid (C18:2). In particular, Aureobasidium sp. accumulated uncommon fatty acids such as petroselinic acid under conditions induced by glycerol. This fatty acid, which has a double bond in position 6,7 and a melting point of 33 °C, highlights its potential as an alternative to margarine production, as well as a precursor to sophorolipids, estolide esters, soaps, and plastics. Rhodotorula sp. exhibited very long-chain fatty acids such as docosadienoic and docosatrienoic acids in its lipid profile. These findings underscore the biotechnological value of yeasts from lentic aquatic systems as sustainable lipid producers, paving the way for innovations in biofuels, nutraceuticals, and oleochemicals.
产油酵母已成为有前景的微生物细胞工厂用于脂质生产，为传统生物柴油和营养保健品来源提供了可持续的替代方案。在本研究中，对从哥伦比亚卡利的两个淡水水生生态系统分离出的酵母菌株的脂质积累潜力进行了评估，以鉴定生物技术应用的新候选菌株。使用以葡萄糖为碳源的重量法脂质测定法对总共56株菌株的产油性质进行了测试。在所评估的菌株中，46.15%的菌株相对于干生物量的脂质产量超过20%。使用甘油作为碳源选择了7株菌株，但仅对5株酵母的脂质谱进行了进一步表征。分子鉴定揭示了多种物种，包括 Aureobasidium sp.、Rajashtanensis 帕皮利奥酵母、红酵母属和葡萄牙棒孢酵母。所选菌株表现出独特的脂质谱，单不饱和脂肪酸和多不饱和脂肪酸比例较高，如油酸（C18:1）和亚油酸（C18:2）。特别是，Aureobasidium sp. 在甘油诱导的条件下积累了不常见的脂肪酸，如岩芹酸。这种脂肪酸在6,7位有一个双键，熔点为33°C，突出了其作为人造黄油生产替代品以及槐糖脂、内酯酯、肥皂和塑料前体的潜力。红酵母属在其脂质谱中表现出非常长链的脂肪酸，如二十二碳二烯酸和二十二碳三烯酸。这些发现强调了来自静水水生系统的酵母作为可持续脂质生产者的生物技术价值，为生物燃料、营养保健品和油脂化学的创新铺平了道路。
