Davis E L, Kaplan D T, Dickson D W, Mitchell D J
J Nematol. 1989 Apr;21(2):219-28.
Treatment of second-stage juveniles (J2) of Meloidogyne incognita race 1 and M. javanica with soybean agglutinin, Concanavalin A, wheat germ agglutinin, Lotus tetragonolobus agglutinin, or Limax flavus agglutinin or the corresponding competitive sugars for each of these lectins did not alter normal root tissue response of soybean cultivars Centennial and Pickett 71 to infection by M. incognita race 1 or M. javanica. Giant cells were frequently induced in Centennial and Pickett 71 roots 5 and 20 days after inoculation of roots with untreated J2 of a population of M. incognita race 3. Treatment of J2 of M. incognita race 3 with the lectins or carbohydrates listed above caused Centennial, but not Pickett 71, root tissue to respond in a hypersensitive manner to infection by M. incognita race 3. Penetration of soybean roots by J2 of Meloidogyne spp. was strongly inhibited in the presence of 0.1 M sialic acid. Treatment of J2 with sialic acid was not lethal to nematodes, and the inhibitory activity of sialic acid was apparently not caused by low pH. These results suggest that carbohydrates may influence plant-nematode interactions.
用大豆凝集素、伴刀豆球蛋白A、麦胚凝集素、百脉根凝集素或黄蛞蝓凝集素，或这些凝集素各自对应的竞争性糖类处理南方根结线虫1号小种和爪哇根结线虫的二期幼虫（J2），并未改变大豆品种百年纪念和皮克特71对南方根结线虫1号小种或爪哇根结线虫感染的正常根组织反应。用南方根结线虫3号小种未经处理的J2接种根系5天和20天后，百年纪念和皮克特71的根中经常诱导形成巨型细胞。用上述凝集素或碳水化合物处理南方根结线虫3号小种的J2，导致百年纪念的根组织对南方根结线虫3号小种的感染产生过敏反应，而皮克特71则没有。在0.1 M唾液酸存在的情况下，根结线虫属J2对大豆根的穿透受到强烈抑制。用唾液酸处理J2对线虫没有致死作用，唾液酸的抑制活性显然不是由低pH引起的。这些结果表明，碳水化合物可能影响植物与线虫的相互作用。
