Melakeberhan H, Webster J M
J Nematol. 1990 Jul;22(3):297-302.
Seven-month-old Scots pine seedlings were inoculated with water or culture filtrate (controls), with 10,000, or 20,000 (experiment 1), and with 2,500 (experiment 2) Bursaphelenchus xylophilus B.C. isolate nematodes and maintained under defined experimental conditions. Controls did not develop pine wilt disease over a 2-month period. In experiment 1, less than 50% of the inoculum was recovered from the nematode-inoculated seedlings in the first 48 hours, after which the nematode population of both treatments increased exponentially resulting in pine death and approximately equal populations at 216 hours after inoculation. In the second experiment, plant mortality, which was always preceded by 2-3 days of chlorosis and associated stem vascular necrosis, first occurred 14 days after inoculation. The nematode population increased until about day 40 after inoculation and declined thereafter. Nematodes extracted from the roots 2 weeks after inoculation accounted for ca.15% of the total number of nematodes per pine. The study indicates that the rate of nematode reproduction is a factor in pine wilt disease. However, the lack of a linear correlation between the number of nematodes and the timing of pine mortality suggests that the timing of pine death may also depend on the location of nematode damage to the host tissue.
对7个月大的苏格兰松幼苗接种水或培养滤液（对照组）、10000条或20000条（实验1）以及2500条（实验2）松材线虫B.C.分离株线虫，并在特定实验条件下进行培育。在2个月的时间里，对照组未出现松材线虫病。在实验1中，接种线虫的幼苗在最初48小时内回收的接种物不到50%，此后两种处理的线虫数量呈指数增长，导致松树死亡，接种后216小时时线虫数量大致相等。在第二个实验中，植物死亡率在接种后14天首次出现，在此之前总是先出现2 - 3天的黄化以及相关的茎干维管束坏死。线虫数量在接种后约40天前增加，此后减少。接种后2周从根部提取的线虫约占每棵松树线虫总数的15%。该研究表明，线虫繁殖率是松材线虫病的一个因素。然而，线虫数量与松树死亡时间之间缺乏线性相关性，这表明松树死亡时间可能还取决于线虫对宿主组织的损伤位置。
