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G-四链体相互作用剂TMPyP4联合光动力疗法对卵巢癌细胞的抗肿瘤活性

Antitumor activity of G-quadruplex-interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells.

作者信息

Liu Hongli, Lv Changshuai, Ding Baijuan, Wang Jie, Li Shan, Zhang Youzhong

机构信息

Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.

出版信息

Oncol Lett. 2014 Jul;8(1):409-413. doi: 10.3892/ol.2014.2125. Epub 2014 May 8.

DOI:10.3892/ol.2014.2125
PMID:24959286
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4063630/
Abstract

The aim of the present study was to investigate the potential effects of photodynamic therapy (PDT) mediated by the cationic porphyrin, 5,10,15,20-tetra-(N-methyl-4-pyridyl)porphine (TMPyP), on an ovarian carcinoma cell line and the underlying mechanisms by which TMPyP-PDT exerts its actions. The analysis of cell viability, hematoxylin and eosin staining and flow cytometric apoptosis assays revealed that TMPyP-PDT potently suppressed the growth of the A2780 cells in a laser energy- and dose-dependent manner. Mechanically, it was observed that TMPyP-PDT suppressed the proliferation and motility of the A2780 cells. In addition, the expression levels of minichromosome maintenance protein-2 (MCM2) and carbonic anhydrase IX (CA-IX) were detected by western blot analysis. The results indicated that the TMPyP-PDT-induced apoptosis and antimetastatic activity in the A2780 cells was accompanied by the inhibition of the expression of MCM2 and CA-IX. Therefore, TMPyP-PDT may represent a potential therapeutic method for the treatment of ovarian carcinoma.

摘要

本研究的目的是探讨由阳离子卟啉5,10,15,20-四（N-甲基-4-吡啶基）卟啉（TMPyP）介导的光动力疗法（PDT）对卵巢癌细胞系的潜在影响以及TMPyP-PDT发挥作用的潜在机制。细胞活力分析、苏木精和伊红染色以及流式细胞术凋亡检测显示，TMPyP-PDT以激光能量和剂量依赖性方式有效抑制A2780细胞的生长。从机制上讲，观察到TMPyP-PDT抑制A2780细胞的增殖和运动。此外，通过蛋白质印迹分析检测微小染色体维持蛋白2（MCM2）和碳酸酐酶IX（CA-IX）的表达水平。结果表明，TMPyP-PDT诱导的A2780细胞凋亡和抗转移活性伴随着MCM2和CA-IX表达的抑制。因此，TMPyP-PDT可能是一种治疗卵巢癌的潜在治疗方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b204/4063630/4b2f7f809aea/OL-08-01-0409-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b204/4063630/3b3f97e99c30/OL-08-01-0409-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b204/4063630/d3f10824a037/OL-08-01-0409-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b204/4063630/fb3a65080db9/OL-08-01-0409-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b204/4063630/58295b397a70/OL-08-01-0409-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b204/4063630/4b2f7f809aea/OL-08-01-0409-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b204/4063630/3b3f97e99c30/OL-08-01-0409-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b204/4063630/d3f10824a037/OL-08-01-0409-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b204/4063630/fb3a65080db9/OL-08-01-0409-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b204/4063630/58295b397a70/OL-08-01-0409-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b204/4063630/4b2f7f809aea/OL-08-01-0409-g04.jpg

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