Mitsumoto Kotaro, Yabusaki Katsumi, Kobayashi Koji, Shirasawa Yoshiaki, Obata Toru
Electronics and Optics Research Laboratories, Kowa Company Ltd., 3-3-1 Chofugaoka, Chofu, Tokyo, Japan.
J Clin Lab Anal. 2009;23(2):117-24. doi: 10.1002/jcla.20300.
Exposure of Limulus amoebocyte lysate to endotoxin under stirring produced light-reflective particles that appeared to be coagulin polymers. A laser light-scattering particle counter, the PA-200, detected these particles sensitively. The PA-200 detected endotoxin at a concentration as low as 0.00015 EU/ml in 71 min, whereas the minimum endotoxin concentration measured by a turbidimeter, ET-2000, was 0.0005 EU/ml in 138 min. Moreover, PA-200 was much less affected by the presence of colored substances and refractive materials than was ET-2000. We propose that the high sensitivity, speed, and high interference tolerance of the laser light-scattering particle-counting method make it more useful than the widely used turbidimetric method for quantitative endotoxin assay.
鲎试剂在搅拌条件下与内毒素接触会产生反光颗粒,这些颗粒似乎是凝固蛋白聚合物。PA - 200型激光散射粒子计数器能够灵敏地检测到这些颗粒。PA - 200在71分钟内就能检测到低至0.00015 EU/ml浓度的内毒素,而用ET - 2000型比浊计测得的最低内毒素浓度在138分钟时为0.0005 EU/ml。此外,与ET - 2000相比,PA - 200受有色物质和折射材料的影响要小得多。我们认为,激光散射粒子计数法的高灵敏度、高速度和高抗干扰性使其比广泛使用的比浊法在定量内毒素检测中更有用。