DuBose D A, Lemaire M, Basamania K, Rowlands J
J Clin Microbiol. 1980 Jan;11(1):68-72. doi: 10.1128/jcm.11.1.68-72.1980.
Due to the presence of inhibitory and possible mimicking substances in plasma difficulties have occurred in the use of the Limulus amoebocyte lysate test. Currently, there are a variety of extraction techniques discussed in the literature which are used to remove these interfering substances, but there is little information comparing these techniques. Five such procedures were compared in their ability to provide an extracted plasma sample in which low levels of endotoxin could be detected by the Limulus amoebocyte lysate test. Results indicated that some procedures adversely affected endotoxin detection. The dilution + heating extraction method was found to be as effective as the widely used chloroform extraction method. Comparison of Limulus amoebocyte lysate test results from healthy human plasma samples extracted by these two methods indicated that lysate type and not extraction procedure was associated with previously reported questionable positive tests. Thus, ambiguities associated with Limulus amoebocyte lysate tests of plasma samples may be due not only to extraction method but also the lysate type employed.
由于血浆中存在抑制性物质以及可能的模拟物质，鲎试剂检测的使用出现了困难。目前，文献中讨论了多种用于去除这些干扰物质的提取技术，但比较这些技术的信息很少。对五种这样的程序在提供可通过鲎试剂检测法检测到低水平内毒素的提取血浆样本的能力方面进行了比较。结果表明，有些程序会对内毒素检测产生不利影响。发现稀释 + 加热提取法与广泛使用的氯仿提取法一样有效。对通过这两种方法提取的健康人血浆样本的鲎试剂检测结果进行比较表明，与先前报道的可疑阳性检测相关的是鲎试剂类型而非提取程序。因此，血浆样本鲎试剂检测相关的模糊性可能不仅归因于提取方法，还归因于所使用的鲎试剂类型。
