鲎凝固蛋白凝胶形成的比浊法研究。
Turbidimetric studies of Limulus coagulin gel formation.
作者信息
Moody T P, Donovan M A, Laue T M
机构信息
Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham 03824, USA.
出版信息
Biophys J. 1996 Oct;71(4):2012-21. doi: 10.1016/S0006-3495(96)79399-2.
The turbidity during trypsin-induced coagulin gel formation was studied over a range of wavelengths. The range of wavelengths used (686-326 nm) also made it possible to investigate the dependence of turbidity on wavelength (the wavelength exponent). Using the results from that work, and structural information on coagulin and the coagulin gel from other studies, a model gel-forming system was designed that consists of species for which the turbidity can be calculated relatively simply. These species include small particles (small in all dimensions relative to the wavelength of incident light); long rods and long random coils (particles that are large in just one dimension relative to the wavelength of incident light); and reflective regions (aggregated material that is large in more than one dimension relative to the wavelength of incident light). The turbidimetric characteristics of the real coagulin gel-forming system are compared with those of the model system.
在一系列波长范围内研究了胰蛋白酶诱导凝固蛋白凝胶形成过程中的浊度。所使用的波长范围（686 - 326 nm）也使得研究浊度对波长的依赖性（波长指数）成为可能。利用该研究的结果以及来自其他研究的关于凝固蛋白和凝固蛋白凝胶的结构信息，设计了一个模型凝胶形成系统，该系统由浊度可相对简单计算的物质组成。这些物质包括小颗粒（在所有维度上相对于入射光波长都很小）；长杆和无规长卷曲（仅在一个维度上相对于入射光波长很大的颗粒）；以及反射区域（相对于入射光波长在多个维度上很大的聚集物质）。将实际凝固蛋白凝胶形成系统的比浊特性与模型系统的比浊特性进行了比较。
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