Wu Kai-Jie, Zeng Jin, Zhu Guo-Dong, Zhang Dong, Xue Yan, Chen Yu-Le, Wang Xin-Yang, He Da-Lin
Department of Urology, The First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
Zhonghua Nan Ke Xue. 2010 Feb;16(2):137-41.
To screen and compare the specific transcription factors that repress the epithelial phenotype in epithelial-mesenchymal transition (EMT) in two different human prostate cancer models LNCaP/HIF1alpha and ARCaP.
We established two different prostate cancer EMT models, LNCaP/HIF1alpha and ARCaP, cultured LNCaP, LNCaP/HIF1alpha, IF11 and IA8 cells in vitro, and detected the five transcription factors Snail, Slug, ZEB1, SIP1 and Twist1 in these cells by RT-PCR.
Different levels of Snail, Slug, ZEB1, SIP1 and Twist1 were detected in both LNCaP and LNCaP/HIF1alpha cells, with significant differences only in the expressions of Slug and Twist1 between the two cells. The expression of Slug was increased, but that of Twist1 decreased in the LNCaP/HIF1alpha cells. All the five transcription factors but Twist1 were expressed in both the IF11 and IA8 cells, but only the express- sions of ZEB1 and Slug were increased significantly in the IA8 cells.
There are different mechanisms underlying transcriptional regulation in different prostate cancer EMT models. Slug may be one of the key transcription factors involved in the HIF1alpha-induced EMT of LNCaP cells, while ZEB1 and Slug may play an important role in repressing the epithelial phenotype of the ARCaP model.
在两种不同的人前列腺癌模型LNCaP/HIF1α和ARCaP中筛选并比较在上皮-间质转化（EMT）过程中抑制上皮表型的特异性转录因子。
我们建立了两种不同的前列腺癌EMT模型，LNCaP/HIF1α和ARCaP，在体外培养LNCaP、LNCaP/HIF1α、IF11和IA8细胞，并通过RT-PCR检测这些细胞中的五种转录因子Snail、Slug、ZEB1、SIP1和Twist1。
在LNCaP和LNCaP/HIF1α细胞中均检测到不同水平的Snail、Slug、ZEB1、SIP1和Twist1，两者之间仅在Slug和Twist1的表达上存在显著差异。在LNCaP/HIF1α细胞中，Slug的表达增加，而Twist1的表达降低。除Twist1外，所有五种转录因子在IF11和IA8细胞中均有表达，但仅在IA8细胞中ZEB1和Slug的表达显著增加。
不同前列腺癌EMT模型中存在不同的转录调控机制。Slug可能是参与HIF1α诱导的LNCaP细胞EMT的关键转录因子之一，而ZEB1和Slug可能在抑制ARCaP模型的上皮表型中起重要作用。
