Nyindo M, Kakoma I, Hansen R
Institute of Primate Research, National Museums of Kenya Karen-Nairobi.
Am J Vet Res. 1991 Aug;52(8):1225-30.
The antigenic profile of Ehrlichia canis, E risticii, E sennetsu, and E equi was investigated by the use of protein (western) immunoblot technique. Results of analysis of serum from acutely and chronically infected animals indicated that the 4 Ehrlichia species share a unique 25-kD polypeptide in addition to other peptides. Immune sera from dogs inoculated with E canis recognized a wide range of E canis polypeptide antigens, as determined by western blot analysis. A larger number of E sennetsu polypeptides were detected when homologous antiserum and antiserum to E equi were used. The latter antiserum did not recognize antigens of E canis or E risticii. Antisera to E canis, E risticii, and E sennetsu detected E equi antigens. Data indicate that a 25-kD protein is a common antigen among the species of the genus Ehrlichia and that the ascending order of abundance of immunodominant determinants in the 4 species of Ehrlichia studied would be: E risticii----E equi----E sennetsu----E canis. Implications of these findings for diagnosis of ehrlichial infections and prophylaxis are evident.
利用蛋白质（western）免疫印迹技术研究了犬埃立克体、瑞氏埃立克体、赛尼茨埃立克体和马埃立克体的抗原谱。对急性和慢性感染动物血清的分析结果表明，除其他肽段外，这4种埃立克体共有一种独特的25-kD多肽。通过western印迹分析确定，接种犬埃立克体的犬的免疫血清可识别多种犬埃立克体多肽抗原。当使用同源抗血清和马埃立克体抗血清时，检测到更多的赛尼茨埃立克体多肽。后一种抗血清不能识别犬埃立克体或瑞氏埃立克体的抗原。犬埃立克体、瑞氏埃立克体和赛尼茨埃立克体的抗血清可检测出马埃立克体抗原。数据表明，25-kD蛋白是埃立克体属各物种之间的共同抗原，在所研究的4种埃立克体中，免疫显性决定簇丰度的升序排列为：瑞氏埃立克体----马埃立克体----赛尼茨埃立克体----犬埃立克体。这些发现对埃立克体感染诊断和预防的意义是显而易见的。
