Ijdo J W, Zhang Y, Anderson M L, Goldberg D, Fikrig E
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-4080, USA.
Clin Diagn Lab Immunol. 1998 Jan;5(1):118-20. doi: 10.1128/CDLI.5.1.118-120.1998.
We describe a patient with human granulocytic ehrlichiosis (HGE), a diagnosis confirmed by PCR and immunoblot analysis. Unexpectedly, immunoglobulin G (IgG) directed towards an 80-kDa ehrlichial antigen (without detectable IgM) was present in the patient's serum in the first week of illness. Lyme disease immunoblots were reactive for IgG (but not IgM), a result indicative of prior exposure to the Lyme disease spirochete. Amino-terminal sequencing revealed that the 80-kDa ehrlichial antigen was an HSP-70 homolog similar to Borrelia burgdorferi HSP-70. We conclude that antibodies against B. burgdorferi HSP-70 may cross-react with the ehrlichial heat shock protein and that this possibility must be considered when serologic test results for HGE and Lyme disease are interpreted.
我们描述了一名患有人类粒细胞埃立克体病（HGE）的患者，其诊断通过聚合酶链反应（PCR）和免疫印迹分析得以证实。出乎意料的是，在该患者发病第一周的血清中就存在针对一种80 kDa埃立克体抗原的免疫球蛋白G（IgG）（未检测到IgM）。莱姆病免疫印迹对IgG呈阳性反应（但对IgM无反应），这一结果表明患者既往曾接触过莱姆病螺旋体。氨基末端测序显示，该80 kDa埃立克体抗原是一种与伯氏疏螺旋体HSP - 70相似的HSP - 70同源物。我们得出结论，针对伯氏疏螺旋体HSP - 70的抗体可能与埃立克体热休克蛋白发生交叉反应，并且在解释HGE和莱姆病的血清学检测结果时必须考虑到这种可能性。
