一种新型人类内源性逆转录病毒的鉴定及其5' U3的启动子活性

Identification of a novel human endogenous retrovirus and promoter activity of its 5' U3.

作者信息

Liang Qiaoyi, Ding Jiayi, Xu Rongzhen, Xu Zefeng, Zheng Shu

机构信息

Cancer Institute, Second Affiliated Hospital, and Ministry of Education Key Laboratory of Cancer Prevention and Intervention, Zhejiang University School of Medicine, Hangzhou, China.

出版信息

Biochem Biophys Res Commun. 2009 May 1;382(2):468-72. doi: 10.1016/j.bbrc.2009.03.058. Epub 2009 Mar 14.

DOI:10.1016/j.bbrc.2009.03.058

PMID:19289097

Abstract

Up-regulation of human endogenous retroviruses (HERVs) sometimes occurs in cancer. This study identifies a novel HERV (HERV-HX) in colon tumor tissues, which is an H family HERV (HERV-H) element located on chromosome Xp22.32. The full-length transcript sequence was identified and characterized. Quantitative RT-PCR indicated it is up-regulated in colon tumor samples. Using RT-PCR to analyze the transcription of the env-deleted HERV-HX and other env-intact HERV-H elements, it was demonstrated that transcription of HERV-H in colon cancer is not associated with the env gene. A 17-bp sequence was found within the 5' U3 region of HERV-HX, and only 8-bp sequences existed on its homologous U3 regions in the corresponding loci. Promoter activity assays indicated that replacing the 17-bp sequence with an 8-bp sequence or deleting it reduced its activity, suggesting that the 17-bp sequence is important to the expression of HERV-HX.

摘要

人类内源性逆转录病毒（HERVs）的上调有时会在癌症中发生。本研究在结肠肿瘤组织中鉴定出一种新型HERV（HERV-HX），它是位于Xp22.32染色体上的H家族HERV（HERV-H）元件。鉴定并表征了全长转录本序列。定量逆转录聚合酶链反应表明它在结肠肿瘤样本中上调。使用逆转录聚合酶链反应分析缺失env的HERV-HX和其他完整env的HERV-H元件的转录，结果表明结肠癌中HERV-H的转录与env基因无关。在HERV-HX 的5' U3区域内发现了一个17 bp的序列，而在相应位点的同源U3区域上仅存在8 bp的序列。启动子活性分析表明，用8 bp的序列替换17 bp的序列或删除它会降低其活性，这表明17 bp的序列对HERV-HX的表达很重要。