Institute for Surgical Research and Hospital Management, University Hospital Basel, Hebelstrasse 20, Basel, Switzerland.
Adv Biochem Eng Biotechnol. 2009;112:1-27. doi: 10.1007/978-3-540-69357-4_1.
In this Chapter we discuss the role of bioreactors in the translational paradigm of Tissue Engineering approaches from basic research to streamlined tissue manufacturing. In particular, we will highlight their functions as: (1) Pragmatic tools for tissue engineers, making up for limitations of conventional manual and static techniques, enabling automation and allowing physical conditioning of the developing tissues; (2) 3D culture model systems, enabling us to recapitulate specific aspects of the actual in vivo milieu and, when properly integrated with computational modeling efforts and sensing and control techniques, to address challenging scientific questions; (3) Tissue manufacturing devices, implementing bioprocesses so as to support safe, standardized, scaleable, traceable and possibly cost-effective production of grafts for clinical use. We will provide evidences that fundamental knowledge gained through the use of well-defined and controlled bio-reactor systems at the research level will be essential to define, optimize, and moreover, streamline the key processes required for efficient manufacturing models.
在本章中，我们讨论了生物反应器在组织工程方法从基础研究到简化组织制造的转化范例中的作用。特别是，我们将强调它们的功能如下：（1）组织工程师的实用工具，弥补了传统手动和静态技术的局限性，实现了自动化，并允许对发育中的组织进行物理调节；（2）3D 培养模型系统，使我们能够再现实际体内环境的具体方面，并且当与计算建模工作以及传感和控制技术适当集成时，能够解决具有挑战性的科学问题；（3）组织制造装置，实施生物工艺，以支持用于临床使用的移植物的安全、标准化、可扩展、可追溯和可能具有成本效益的生产。我们将提供证据表明，通过在研究水平上使用定义明确和控制良好的生物反应器系统获得的基础知识对于定义、优化，而且，简化高效制造模型所需的关键过程将是至关重要的。
