Souppart Laetitia, Sanciu Giovanna, Cian Amandine, Wawrzyniak Ivan, Delbac Frederic, Capron Monique, Dei-Cas Eduardo, Boorom Kenneth, Delhaes Laurence, Viscogliosi Eric
Inserm, U547, Institut Pasteur de Lille, Université Lille Nord de France, 1 rue du Professeur Calmette, BP 245, 59019, Lille Cedex, France.
Parasitol Res. 2009 Aug;105(2):413-21. doi: 10.1007/s00436-009-1398-9. Epub 2009 Mar 17.
Blastocystis sp. is the most common eukaryotic parasite in the intestinal tract of humans. Due to its strong impact in public health, in this study, we determined the frequency of different Blastocystis subtypes in patients in France. We hypothesized on the mode of transmission and tested a possible relationship between the subtype and symptomatic status. We obtained a total of 40 stool samples identified as positive for Blastocystis by microscopic examination of smears. Participants consisted of 25 symptomatic and 15 asymptomatic patients, for whom clinical and parasitological data were collected. For nested-polymerase chain reaction and genotyping, DNA was extracted directly from fecal samples or from fecal cultures. Morphological forms observed in fecal cultures were uncorrelated with symptomatic status. Genotyping using partial small subunit rRNA gene analysis identified a total of 43 Blastocystis isolates corresponding to 37 single infections and three mixed infections by two different subtypes. These 43 isolates belonged to five subtypes (1, 2, 3, 4, and 7) with predominance of subtype 3 (53.5%). Patient symptomatic status was uncorrelated with Blastocystis subtype.
芽囊原虫是人类肠道中最常见的真核寄生虫。鉴于其对公共卫生有重大影响，在本研究中，我们测定了法国患者中不同芽囊原虫亚型的出现频率。我们对其传播方式进行了假设，并测试了亚型与症状状态之间可能存在的关系。通过涂片显微镜检查，我们共获得了40份被鉴定为芽囊原虫阳性的粪便样本。参与者包括25名有症状患者和15名无症状患者，我们收集了他们的临床和寄生虫学数据。对于巢式聚合酶链反应和基因分型，直接从粪便样本或粪便培养物中提取DNA。在粪便培养物中观察到的形态形式与症状状态无关。使用部分小亚基rRNA基因分析进行基因分型，共鉴定出43株芽囊原虫分离株，其中37株为单一感染，3株为两种不同亚型的混合感染。这43株分离株属于5种亚型（1、2、3、4和7），其中亚型3占主导地位（53.5%）。患者的症状状态与芽囊原虫亚型无关。
