Wu Song, Pan Jia-Yun, Wang Xue-Zhong, Zhou Shui-Sen, Zhang Guo-Qing, Liu Qian, Tang Lin-Hua
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, PR China.
Malar J. 2009 Mar 16;8:46. doi: 10.1186/1475-2875-8-46.
Malaria is endemic in Linzhi Prefecture in the Tibet Autonomous Region (TAR), but the vector for malaria transmission had never been identified.
Adult Anopheles spp. were collected in Motuo County, Linzhi Prefecture on the Sino-Indian border in July and August, 2007. Multiplex PCR was adopted for species identification, and a nested PCR approach was used to detect sporozoites in the salivary glands of the mosquitoes.
3,675 mosquitoes of the Anopheles maculatus group were collected and processed for species identification. Among them, 3,602 (98.0%) were Anopheles pseudowillmori and 73 (2.0%) were Anopheles willmori. The Plasmodium vivax SSUrDNA fragment was amplified in two of 360 pooled An. pseudowillmori samples.
The local An. maculatus group comprises the species An. pseudowillmori and An. willmori. Anopheles pseudowillmori is considered the sole malaria vector in Motuo County in Linzhi Prefecture.
疟疾在西藏自治区林芝地区呈地方性流行,但疟疾传播媒介一直未被确定。
2007年7月和8月,在中印边境的林芝地区墨脱县采集成年按蚊。采用多重PCR进行种类鉴定,并用巢式PCR方法检测蚊子唾液腺中的子孢子。
共采集3675只斑腿按蚊复合体的蚊子并进行种类鉴定。其中,3602只(98.0%)为伪威氏按蚊,73只(2.0%)为威氏按蚊。在360份混合的伪威氏按蚊样本中的两份中扩增出间日疟原虫小亚基核糖体DNA片段。
当地的斑腿按蚊复合体包括伪威氏按蚊和威氏按蚊。伪威氏按蚊被认为是林芝地区墨脱县唯一的疟疾传播媒介。