Sevc Juraj, Daxnerová Zuzana, Miklosová Mária
Institute of Biology and Ecology, Faculty of Sciences, PJ Safárik University, 04167 Kosice, Slovak Republic.
Cell Mol Neurobiol. 2009 Sep;29(6-7):927-36. doi: 10.1007/s10571-009-9377-3. Epub 2009 Mar 17.
In the last quarter of the embryonic development of rat and shortly after a termination of neurogenesis, the transformation of the spinal cord primitive lumen (pL) to the central canal (CC) occurs. In this work, we show that this phenomenon is not an insignificant event but it is directly associated with the processes of gliogenesis. Using a light microscopy and immunohistochemistry, we monitored the development of the rat embryonic spinal cord from the end of the neurogenesis on the embryonic day 17 until the maturation of the spinal cord during the first postnatal weeks. Our observations demonstrate the importance of the transformation of the pL to the CC and its connection with gliogenesis, and the mechanism of this transformation is proposed. It is found that a segregation of the glutamate transporter (GLAST) immunopositive cells from the alar plates and transformation of the radial glial cells to the fibrous and protoplasmic astrocytes play presumably a key role in the diminution of the ventricular zone. Results indicate that the very transformation and migration of the radial glial cells during gliogenesis could result in a transformation of the pL to the CC.
在大鼠胚胎发育的最后一个季度以及神经发生终止后不久，脊髓原始管腔（pL）会转变为中央管（CC）。在这项研究中，我们表明这一现象并非微不足道，而是与神经胶质生成过程直接相关。我们使用光学显微镜和免疫组织化学方法，监测了从胚胎第17天神经发生结束时起大鼠胚胎脊髓的发育情况，直至出生后第一周脊髓成熟。我们的观察结果证明了pL向CC转变的重要性及其与神经胶质生成的联系，并提出了这种转变的机制。研究发现，谷氨酸转运体（GLAST）免疫阳性细胞从翼板分离以及放射状胶质细胞向纤维性和原浆性星形胶质细胞的转变，可能在脑室区缩小过程中起关键作用。结果表明，在神经胶质生成过程中放射状胶质细胞的这种转变和迁移可能导致pL向CC的转变。
