Xu Zhi, Zhang Fan, Xu Bosong, Tan Jingze, Li Shilin, Jin Li
State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes of Biomedical Sciences, Fudan University, Shanghai, PR China.
Electrophoresis. 2009 Apr;30(8):1282-5. doi: 10.1002/elps.200800473.
Much attention has been paid on ancient DNA (aDNA) studies, and negative control was used as one of the stringent quality assurance criteria in order to detect potential contamination. However, the results of some aDNA studies showed the evidence of contamination despite their negative controls failed to do so. Using lambda DNA to mock extraneous contaminating DNA, our study showed that aDNA had a property of improving the efficiency of extraction including contaminating DNA, while negative controls had low sensitivity to detect contamination. To circumvent this problem, carrier DNA such as poly(dA) is suggested to be introduced into aDNA extraction.
古代DNA（aDNA）研究已受到广泛关注，阴性对照被用作严格的质量保证标准之一，以检测潜在的污染。然而，一些aDNA研究的结果显示出污染的迹象，尽管其阴性对照未检测到污染。我们的研究使用λDNA模拟外来污染DNA，结果表明aDNA具有提高包括污染DNA在内的提取效率的特性，而阴性对照对检测污染的敏感性较低。为了解决这个问题，建议将诸如聚（dA）等载体DNA引入aDNA提取中。
