大肠杆菌八聚体外膜蛋白Wza的脉冲电子双共振光谱距离指纹分析
PELDOR spectroscopy distance fingerprinting of the octameric outer-membrane protein Wza from Escherichia coli.
作者信息
Hagelueken Gregor, Ingledew W John, Huang Hexian, Petrovic-Stojanovska Biljana, Whitfield Chris, ElMkami Hassane, Schiemann Olav, Naismith James H
机构信息
Centre for Biomolecular Sciences, The University of St. Andrews, Fife KY16 9RH, UK.
出版信息
Angew Chem Int Ed Engl. 2009;48(16):2904-6. doi: 10.1002/anie.200805758.
Abstract
Distance fingerprinting: Pulsed electron-electron double resonance spectroscopy (PELDOR) is applied to the octameric membrane protein complex Wza of E. coli. The data yielded a detailed distance fingerprint of its periplasmic region that compares favorably to the crystal structure. These results provide the foundation to study conformation changes from interaction with partner proteins.
摘要
距离指纹图谱
脉冲电子-电子双共振光谱法（PELDOR）应用于大肠杆菌的八聚体膜蛋白复合物Wza。数据产生了其周质区域的详细距离指纹图谱，与晶体结构相比具有优势。这些结果为研究与伴侣蛋白相互作用引起的构象变化奠定了基础。
相似文献
1
PELDOR spectroscopy distance fingerprinting of the octameric outer-membrane protein Wza from Escherichia coli.大肠杆菌八聚体外膜蛋白Wza的脉冲电子双共振光谱距离指纹分析
Angew Chem Int Ed Engl. 2009;48(16):2904-6. doi: 10.1002/anie.200805758.
2
Translocation of group 1 capsular polysaccharide in Escherichia coli serotype K30. Structural and functional analysis of the outer membrane lipoprotein Wza.1型荚膜多糖在大肠杆菌血清型K30中的转运。外膜脂蛋白Wza的结构与功能分析。
J Biol Chem. 2003 Dec 12;278(50):49763-72. doi: 10.1074/jbc.M308775200. Epub 2003 Sep 30.
3
Modeling structural transitions from the periplasmic-open state of lactose permease and interpretations of spin label experiments.乳糖通透酶周质开放状态的结构转变建模及自旋标记实验解读
Biochim Biophys Acta. 2016 Jul;1858(7 Pt A):1541-52. doi: 10.1016/j.bbamem.2016.04.008. Epub 2016 Apr 20.
4
Structure and function of the Escherichia coli Tol-Pal stator protein TolR.大肠杆菌Tol-Pal定子蛋白TolR的结构与功能
J Biol Chem. 2015 Oct 30;290(44):26675-87. doi: 10.1074/jbc.M115.671586. Epub 2015 Sep 9.
5
Ligand Induced Conformational Changes of a Membrane Transporter in E. coli Cells Observed with DEER/PELDOR.用双电子-电子共振/脉冲电子双共振观测大肠杆菌细胞中膜转运蛋白的配体诱导构象变化
J Am Chem Soc. 2016 Feb 17;138(6):1844-7. doi: 10.1021/jacs.5b13382. Epub 2016 Feb 2.
6
In situ observation of conformational dynamics and protein ligand-substrate interactions in outer-membrane proteins with DEER/PELDOR spectroscopy.利用双电子共振/偏振滤波相关光谱法在外膜蛋白中进行构象动力学和蛋白质配体-底物相互作用的原位观察。
Nat Protoc. 2019 Aug;14(8):2344-2369. doi: 10.1038/s41596-019-0182-2. Epub 2019 Jul 5.
7
Three-dimensional structure of Wza, the protein required for translocation of group 1 capsular polysaccharide across the outer membrane of Escherichia coli.Wza的三维结构，Wza是1型荚膜多糖穿过大肠杆菌外膜所必需的蛋白质。
J Biol Chem. 2004 Jul 2;279(27):28227-32. doi: 10.1074/jbc.M402913200. Epub 2004 Apr 16.
8
Structure-function relationships of the outer membrane translocon Wza investigated by cryo-electron microscopy and mutagenesis.通过冷冻电子显微镜和诱变研究外膜转运体Wza的结构-功能关系。
J Struct Biol. 2009 May;166(2):172-82. doi: 10.1016/j.jsb.2009.02.005. Epub 2009 Feb 21.
9
Crystallization and preliminary X-ray diffraction analysis of Wza outer-membrane lipoprotein from Escherichia coli serotype O9a:K30.大肠杆菌O9a:K30血清型Wza外膜脂蛋白的结晶及初步X射线衍射分析
Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):558-60. doi: 10.1107/S0907444903029494. Epub 2004 Feb 25.
10
Structure of TonB in complex with FhuA, E. coli outer membrane receptor.与大肠杆菌外膜受体FhuA结合的TonB的结构
Science. 2006 Jun 2;312(5778):1399-402. doi: 10.1126/science.1128057.
引用本文的文献
1
Darobactin B Stabilises a Lateral-Closed Conformation of the BAM Complex in Cells.达罗巴汀B在细胞中稳定了BAM复合物的侧向封闭构象。
Angew Chem Weinheim Bergstr Ger. 2023 Aug 21;135(34):e202218783. doi: 10.1002/ange.202218783. Epub 2023 May 31.
2
Pulse Dipolar Electron Paramagnetic Resonance Spectroscopy Distance Measurements at Low Nanomolar Concentrations: The Cu-Trityl Case.低纳摩尔浓度下的脉冲偶极电子顺磁共振光谱距离测量：铜-三芳甲基自由基的情况
J Phys Chem Lett. 2024 Feb 8;15(5):1455-1461. doi: 10.1021/acs.jpclett.3c03311. Epub 2024 Jan 31.
3
Darobactin B Stabilises a Lateral-Closed Conformation of the BAM Complex in E. coli Cells.达罗巴辛 B 稳定了 BAM 复合物在大肠杆菌细胞中的侧向闭合构象。
Angew Chem Int Ed Engl. 2023 Aug 21;62(34):e202218783. doi: 10.1002/anie.202218783. Epub 2023 May 31.
4
Investigating Native Metal Ion Binding Sites in Mammalian Histidine-Rich Glycoprotein.研究哺乳动物组氨酸丰富糖蛋白中的天然金属离子结合位点。
J Am Chem Soc. 2023 Apr 12;145(14):8064-8072. doi: 10.1021/jacs.3c00587. Epub 2023 Mar 31.
5
The making of hypervirulent Klebsiella pneumoniae.产超毒力肺炎克雷伯菌的形成。
J Clin Lab Anal. 2022 Dec;36(12):e24743. doi: 10.1002/jcla.24743. Epub 2022 Nov 8.
6
Dipolar pathways in multi-spin and multi-dimensional dipolar EPR spectroscopy.双自旋和多维双极电子顺磁共振波谱中的偶极途径。
Phys Chem Chem Phys. 2022 Sep 28;24(37):22645-22660. doi: 10.1039/d2cp03048a.
7
Pulse dipolar EPR for determining nanomolar binding affinities.用于测定纳摩尔结合亲和力的脉冲双极 EPR。
Chem Commun (Camb). 2022 Aug 4;58(63):8790-8793. doi: 10.1039/d2cc02360a.
8
PELDOR Measurements on Nitroxide-Labeled Oligonucleotides.PELDOR 测量技术在氮氧自由基标记寡核苷酸中的应用。
Methods Mol Biol. 2022;2439:241-274. doi: 10.1007/978-1-0716-2047-2_16.
9
Benchmark Test and Guidelines for DEER/PELDOR Experiments on Nitroxide-Labeled Biomolecules.用于氮氧自由基标记生物分子的 DEER/PELDOR 实验的基准测试和指南。
J Am Chem Soc. 2021 Nov 3;143(43):17875-17890. doi: 10.1021/jacs.1c07371. Epub 2021 Oct 19.
10
Spin-labelled mechanically interlocked molecules as models for the interpretation of biradical EPR spectra.自旋标记的机械互锁分子作为解释双自由基电子顺磁共振谱的模型。
Chem Sci. 2021 May 14;12(24):8385-8393. doi: 10.1039/d1sc01462e.
本文引用的文献
1
Phaser crystallographic software.相位结晶学软件。
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674. doi: 10.1107/S0021889807021206. Epub 2007 Jul 13.
2
A comparative electron paramagnetic resonance study of the nucleotide-binding domains' catalytic cycle in the assembled maltose ATP-binding cassette importer.组装型麦芽糖ATP结合盒转运体中核苷酸结合结构域催化循环的比较电子顺磁共振研究。
Biophys J. 2008 Sep 15;95(6):2924-38. doi: 10.1529/biophysj.108.132456. Epub 2008 Jun 20.
3
Periplasmic export machines for outer membrane assembly.用于外膜组装的周质输出机器。
Curr Opin Struct Biol. 2008 Aug;18(4):466-74. doi: 10.1016/j.sbi.2008.04.001. Epub 2008 May 19.
4
Structural determinants of nitroxide motion in spin-labeled proteins: solvent-exposed sites in helix B of T4 lysozyme.自旋标记蛋白质中氮氧化物运动的结构决定因素：T4溶菌酶螺旋B中的溶剂暴露位点。
Protein Sci. 2008 Feb;17(2):228-39. doi: 10.1110/ps.073174008. Epub 2007 Dec 20.
5
High-resolution structure of a Na+/H+ antiporter dimer obtained by pulsed electron paramagnetic resonance distance measurements.通过脉冲电子顺磁共振距离测量获得的Na⁺/H⁺反向转运蛋白二聚体的高分辨率结构。
Biophys J. 2007 Nov 15;93(10):3675-83. doi: 10.1529/biophysj.107.109769. Epub 2007 Aug 17.
6
Long-range distance determinations in biomacromolecules by EPR spectroscopy.利用电子顺磁共振波谱法测定生物大分子中的远程距离
Q Rev Biophys. 2007 Feb;40(1):1-53. doi: 10.1017/S003358350700460X. Epub 2007 Jun 13.
7
Counting the monomers in nanometer-sized oligomers by pulsed electron-electron double resonance.通过脉冲电子-电子双共振对纳米级低聚物中的单体进行计数。
J Am Chem Soc. 2007 May 30;129(21):6736-45. doi: 10.1021/ja065787t. Epub 2007 May 8.
8
Wza the translocon for E. coli capsular polysaccharides defines a new class of membrane protein.大肠杆菌荚膜多糖的转运体Wza定义了一类新的膜蛋白。
Nature. 2006 Nov 9;444(7116):226-9. doi: 10.1038/nature05267. Epub 2006 Nov 1.
9
Biosynthesis and assembly of capsular polysaccharides in Escherichia coli.大肠杆菌中荚膜多糖的生物合成与组装
Annu Rev Biochem. 2006;75:39-68. doi: 10.1146/annurev.biochem.75.103004.142545.
10
Two-step purification of outer membrane proteins.外膜蛋白的两步纯化
Int J Biol Macromol. 2006 Aug 15;39(1-3):10-4. doi: 10.1016/j.ijbiomac.2005.12.008. Epub 2006 Jan 19.
Zotero 插件