Mendes N D, Freitas A T, Sagot M-F
Equipe BAOBAB, Laboratoire de Biométrie et Biologie Evolutive (UMR 5558), CNRS, Univ. Lyon 1, 43 bd du 11 nov 1918, 69622, Villeurbanne Cedex, France.
Nucleic Acids Res. 2009 May;37(8):2419-33. doi: 10.1093/nar/gkp145. Epub 2009 Mar 18.
The discovery of microRNAs (miRNAs), almost 10 years ago, changed dramatically our perspective on eukaryotic gene expression regulation. However, the broad and important functions of these regulators are only now becoming apparent. The expansion of our catalogue of miRNA genes and the identification of the genes they regulate owe much to the development of sophisticated computational tools that have helped either to focus or interpret experimental assays. In this article, we review the methods for miRNA gene finding and target identification that have been proposed in the last few years. We identify some problems that current approaches have not yet been able to overcome and we offer some perspectives on the next generation of computational methods.
大约10年前，微小RNA（miRNA）的发现极大地改变了我们对真核基因表达调控的看法。然而，这些调控因子广泛而重要的功能直到现在才逐渐显现出来。我们miRNA基因目录的扩充以及它们所调控基因的鉴定在很大程度上得益于先进计算工具的发展，这些工具有助于聚焦或解释实验分析。在本文中，我们回顾了过去几年中提出的miRNA基因发现和靶标鉴定方法。我们指出了当前方法尚未能克服的一些问题，并对下一代计算方法提供了一些观点。
