Zhang X M, Horiguchi Y, Ueda M, Yoshiki T, Imamura S
Department of Dermatology, Faculty of Medicine, Kyoto University, Japan.
Arch Dermatol Res. 1991;283(5):310-6. doi: 10.1007/BF00376619.
Immunohistochemical and immunochemical analyses were performed on a monoclonal antibody designated 1-2B7B which was derived from immunizing mice with human prostate epithelial tissue. The 1-2B7B antigen was expressed not only along the acinous basement membrane zone (BMZ) of the prostate and testis, but also along the BMZ of the epithelia of several other organs including the skin, oesophagus, urinary bladder, ureter, stomach, intestine and bile duct. The antigenic epitope was not expressed in these tissues of lower mammals. Immunoelectron microscopic studies on normal human skin revealed that the 1-2B7B antigen was localized mainly just beneath the hemidesmosomes of basal keratinocytes, but not beneath melanocytes. Indirect immunofluorescence and immunoelectron microscopic studies on 1 M NaCl-split skin confirmed that this antigen was not separated from the cytoplasmic membrane of basal cells after salt treatment. SDS polyacrylamide gel electrophoresis of immunochemically purified protein from the epidermis demonstrated the molecular weight of the antigen to be 120 kDa. 1-2B7B monoclonal antibody should be a useful probe for studying the pathomechanism of some blistering diseases, as well as the assembly and function of the epidermal-dermal junction.
对一种名为1-2B7B的单克隆抗体进行了免疫组织化学和免疫化学分析，该抗体是通过用人前列腺上皮组织免疫小鼠获得的。1-2B7B抗原不仅在前列腺和睾丸的腺泡基底膜区（BMZ）表达，还在包括皮肤、食道、膀胱、输尿管、胃、肠道和胆管在内的其他几个器官上皮的BMZ表达。该抗原表位在低等哺乳动物的这些组织中不表达。对正常人皮肤的免疫电子显微镜研究表明，1-2B7B抗原主要定位于基底角质形成细胞半桥粒下方，而不是黑素细胞下方。对经1 M NaCl处理的皮肤进行间接免疫荧光和免疫电子显微镜研究证实，盐处理后该抗原未与基底细胞的细胞质膜分离。对表皮免疫化学纯化蛋白进行的SDS聚丙烯酰胺凝胶电泳显示该抗原的分子量为120 kDa。1-2B7B单克隆抗体应是研究某些水疱性疾病发病机制以及表皮-真皮连接组装和功能的有用探针。
