Xu Jin, Yang Y, Wang C, Jiang B
Pediatric Institute, Children's Hospital, Fudan University, 183 Fenglin Road, 200032, Shanghai, China.
Inflamm Res. 2009 Sep;58(9):585-92. doi: 10.1007/s00011-009-0022-x. Epub 2009 Mar 19.
To understand the inflammatory-immune response in intestinal epithelial cells after infection of rotavirus and coxsackievirus B3.
We examined by quantitative PCR the expression profiles of genes encoding five toll-like receptors (TLR) and levels of three chemokines in response to rotavirus and coxsackievirus B3 infection in a human intestinal epithelial cell line (HT-29 cells).
We demonstrated that rotavirus induced significantly increased levels of mRNA expression for TLR2, TLR3, TLR7 and TLR8 in HT-29 cells in a time-dependent manner. In contrast, coxsackievirus B3 did not stimulate mRNA expression for TLR3. Rotavirus and coxsackievirus B3 also induced higher levels of mRNA expression for RANTES, IP-10 and IL-8 during the period of infection in a different manner. Finally, significantly elevated levels of RANTES, IP-10 and IL-8 were detected by ELISA in rotavirus-infected cells from 24 to 48 h.
Our findings suggest that different patterns of TLRs and chemokines were induced in the initiation and modulation of immune response to rotavirus and coxsackievirus B3 infection.
了解轮状病毒和柯萨奇病毒B3感染后肠上皮细胞中的炎症免疫反应。
我们通过定量PCR检测了人类肠上皮细胞系（HT-29细胞）中编码五种Toll样受体（TLR）的基因表达谱以及三种趋化因子在轮状病毒和柯萨奇病毒B3感染后的水平。
我们证明，轮状病毒以时间依赖性方式显著增加了HT-29细胞中TLR2、TLR3、TLR7和TLR8的mRNA表达水平。相比之下，柯萨奇病毒B3并未刺激TLR3的mRNA表达。轮状病毒和柯萨奇病毒B3在感染期间也以不同方式诱导了RANTES、IP-10和IL-8的更高水平的mRNA表达。最后，通过ELISA检测发现，在24至48小时期间，轮状病毒感染的细胞中RANTES、IP-10和IL-8的水平显著升高。
我们的研究结果表明，在对轮状病毒和柯萨奇病毒B3感染的免疫反应的启动和调节中，诱导了不同模式的TLR和趋化因子。
