Vilcins Inger-Marie E, Old Julie M, Deane Elizabeth
Department of Biological Sciences, E8A, Macquarie University, Sydney, NSW, 2109, Australia.
Exp Appl Acarol. 2009 Nov;49(3):229-42. doi: 10.1007/s10493-009-9260-4. Epub 2009 Mar 19.
Three Australian native animal species yielded 60 samples composed of three indigenous ticks. Hosts included twelve koalas, two echidnas and one wombat from Victoria, and ticks were of the species Ixodes tasmani (n = 42), Bothriocroton concolor (n = 8) and B. auruginans (n = 10), respectively. PCR screening and sequencing detected a species of Coxiella, sharing closest sequence identity to C. burnetii (>98%), in all B. auruginans, as well as a species of Rickettsia, matching closest to R. massiliae, in 70% of the same samples. A genotype sharing closest similarity to Rickettsia bellii (>99%) was identified in three female B. concolor collected from one of the echidnas. Three samples of I. tasmani, taken from three koalas, yielded different genotypes of Rickettsiella. These results represent the first detection of the three genera in each tick species and identify a high level of previously undetected bacterial diversity in Australian ticks.
三种澳大利亚本土动物物种提供了由三种本土蜱虫组成的60个样本。宿主包括来自维多利亚州的12只树袋熊、2只针鼹和1只袋熊，蜱虫分别为塔斯马尼亚硬蜱（n = 42）、同色牛蜱（n = 8）和铜绿牛蜱（n = 10）。PCR筛查和测序在所有铜绿牛蜱中检测到一种柯克斯体，其与伯纳特柯克斯体的序列同一性最接近（>98%），并且在70%的相同样本中检测到一种立克次体，与马赛立克次体最匹配。在从一只针鼹身上采集的三只雌性同色牛蜱中鉴定出一种与贝利立克次体相似度最高（>99%）的基因型。从三只树袋熊身上采集的三个塔斯马尼亚硬蜱样本产生了不同基因型的立克次体属细菌。这些结果代表了在每种蜱虫物种中首次检测到这三个属，并确定了澳大利亚蜱虫中存在高水平的先前未被检测到的细菌多样性。
