The Centre for Biomedical Engineering, Institute of Orthopaedics and Musculoskeletal Science, University College London, Stanmore, Middlesex, United Kingdom.
J Biomed Mater Res A. 2010 Mar 1;92(3):1028-37. doi: 10.1002/jbm.a.32450.
The success of intraosseous transcutaneous amputation prostheses (ITAP) relies on soft tissue attachment to prevent infection which leads to implant failure. Fibronectin (Fn) has been shown to enhance dermal fibroblast attachment in vitro, however measurement of cell attachment strength has been indirect; using cell area and immunolocalization of focal adhesion components. In this study, we have developed a flow apparatus to assess the biophysical strength of cell attachment to biomaterials used in ITAP. We have demonstrated that dermal fibroblast attachment strength increases significantly up to 96 h and that data from direct and indirect methods of assessing cell attachment strength have a significant positive correlation. Additionally, we have used direct and indirect assessment methods to demonstrate that dermal fibroblast attachment strength is significantly greater on fibronectin-coated titanium alloy compared with uncoated controls at 1, 4, and 24 hours.
经皮穿骨截肢假体（ITAP）的成功依赖于软组织附着以防止感染导致植入物失败。纤连蛋白（Fn）已被证明可增强体外真皮成纤维细胞的附着，但细胞附着强度的测量一直是间接的，使用细胞面积和焦点粘连成分的免疫定位。在这项研究中，我们开发了一种流动装置来评估 ITAP 中使用的生物材料的细胞附着的生物物理强度。我们已经证明，真皮成纤维细胞附着强度在 96 小时内显著增加，并且直接和间接评估细胞附着强度的方法的数据具有显著的正相关。此外，我们还使用直接和间接评估方法证明，与未涂层对照相比，在 1、4 和 24 小时时，真皮成纤维细胞在纤连蛋白涂覆的钛合金上的附着强度显著更高。
