Maille L, Bergere M, Lemoine E, Camier B, Prevost J F, Bourdrel J M, Hammoud I, Selva J, Vialard F
Laboratoire Albert 1er and Clinique St Thérèse, 80000 Amiens, France.
Reprod Biomed Online. 2009 Mar;18(3):367-73. doi: 10.1016/s1472-6483(10)60095-8.
Pronuclear morphology has been reported as a good tool for studying embryo development and euploidy. Comparing two groups of women with different aneuploidy risk, women more than 38 years old (n = 28) known to be at high risk of aneuploidy, and women under 30 years old (n = 35), this study investigated whether pronuclear morphology could be used routinely as an alternative to preimplantation genetic screening (PGS) in countries where PGS is prohibited. Pronuclear morphology was evaluated for 301 zygotes and related to embryo quality and pregnancy outcome. For the older women, an increased frequency of zygotes with abnormal polar body and pronuclei alignment was observed, i.e. type gamma, with 93% aneuploidy risk (26.0 versus 15.1% P < 0.05) and fewer zygotes with a good development prognosis (36.4 versus 47.8%; P < 0.05). A1alpha configuration was associated with good implantation rate and was not related to day 2 embryo quality. This configuration was less frequent in the group of women more than 38 years old and among non-pregnant women under 30 years, compared with pregnant women under 30 years old. Pronuclear morphology seemed linked to age, but not associated with embryo quality. A larger study allowing correlation analysis is necessary to confirm the value of these criteria and the link to a woman's age.
原核形态已被报道为研究胚胎发育和整倍体的良好工具。本研究比较了两组非整倍体风险不同的女性，一组是已知非整倍体风险高的38岁以上女性（n = 28），另一组是30岁以下女性（n = 35），调查了在禁止植入前基因筛查（PGS）的国家，原核形态是否可以常规用作PGS的替代方法。对301个受精卵的原核形态进行了评估，并将其与胚胎质量和妊娠结局相关联。对于年龄较大的女性，观察到极体和原核排列异常的受精卵频率增加，即γ型，非整倍体风险为93%（26.0%对15.1%，P < 0.05），而发育预后良好的受精卵较少（36.4%对47.8%；P < 0.05）。A1α构型与良好的着床率相关，且与第2天的胚胎质量无关。与30岁以下的孕妇相比，这种构型在38岁以上的女性组和30岁以下的未怀孕女性中频率较低。原核形态似乎与年龄有关，但与胚胎质量无关。需要进行更大规模的研究以进行相关性分析，以证实这些标准的价值以及与女性年龄的关联。
