红球菌属 Y22 的分离、鉴定及其在烟草加工中的潜在应用。
Isolation and characterization of Rhodococcus sp. Y22 and its potential application to tobacco processing.
作者信息
Gong Xiao-Wei, Yang Jin-Kui, Duan Yan-Qin, Dong Jin-Yan, Zhe Wei, Wang Le, Li Qing-Hua, Zhang Ke-Qin
机构信息
Laboratory for Conservation and Utilization of Bio-resources, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, People's Republic of China.
出版信息
Res Microbiol. 2009 Apr;160(3):200-4. doi: 10.1016/j.resmic.2009.02.004. Epub 2009 Mar 17.
A novel nicotine-degrading bacterium, strain Y22, was isolated and identified as Rhodococcus sp. Y22 based on its 16S rDNA sequence and morphological and biochemical features. The isolate could utilize nicotine as the sole source of carbon and nitrogen. Nicotine (1.0g/L) was degraded by Rhodococcus sp. Y22 within 52h at 28 degrees C and pH 7.0. Preparation of resting cells from nicotine-induced cultures was found to rapidly and efficiently degrade nicotine from solutions as well as from tobacco leaves. Therefore, Rhodococcus sp. Y22 has the potential to degrade nicotine during tobacco leave processing.
分离出一种新型尼古丁降解细菌菌株Y22，根据其16S rDNA序列以及形态和生化特征，鉴定为红球菌属Y22。该分离株能够利用尼古丁作为唯一的碳源和氮源。在28℃和pH 7.0条件下，红球菌属Y22在52小时内可降解1.0g/L的尼古丁。研究发现，由尼古丁诱导培养物制备的静息细胞能够快速有效地降解溶液以及烟草叶片中的尼古丁。因此，红球菌属Y22在烟叶加工过程中具有降解尼古丁的潜力。
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