Mohamed Ahmed Isam Ali, Arima Jiro, Ichiyanagi Tsuyoshi, Sakuno Emi, Mori Nobuhiro
United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan.
FEMS Microbiol Lett. 2009 Jun;296(2):219-25. doi: 10.1111/j.1574-6968.2009.01641.x. Epub 2009 May 6.
The aerobic degradation of 3-N-trimethylamino-1-propanol (homocholine) as a sole source of carbon and nitrogen has been found for a Rhodococcus sp. bacterium isolated from soil. The isolate was identified as Rhodococcus sp. strain A2 based on its phenotypic features, physiological and biochemical characteristics, and results of phylogenetic analysis. The washed cells of strain A2 completely degraded homocholine within 6 h, with concomitant formation of several metabolites. Analysis of the metabolites using capillary electrophoresis, fast atom bombardment-MS, and GC-MS showed that trimethylamine was the major metabolite, in addition to beta-alanine betaine (beta-AB) and trimethylaminopropionaldehyde. Therefore, the possible degradation pathway of homocholine in the isolated strain is through consequent oxidation of the alcohol group (-OH) to aldehyde (-CHO) and acid (-COOH). Thereafter, the cleavage of beta-AB C-N bonds yielded trimethylamine and alkyl chain.
从土壤中分离出的一株红球菌属细菌能够以3-N-三甲基氨基-1-丙醇（高胆碱）作为唯一碳源和氮源进行好氧降解。根据其表型特征、生理生化特性以及系统发育分析结果，该分离菌株被鉴定为红球菌属菌株A2。菌株A2的洗涤细胞在6小时内完全降解了高胆碱，并同时形成了几种代谢产物。使用毛细管电泳、快原子轰击质谱和气相色谱-质谱对代谢产物进行分析表明，除了β-丙氨酸甜菜碱（β-AB）和三甲基氨基丙醛外，三甲胺是主要代谢产物。因此，分离菌株中高胆碱可能的降解途径是通过醇基（-OH）依次氧化为醛基（-CHO）和羧基（-COOH）。此后，β-AB中C-N键的断裂产生了三甲胺和烷基链。
