Pati Sandra, Liberatore Maria Teresa, Gambacorta Giuseppe, Antonacci Donato, La Notte Ennio
Department of Food Science, University of Foggia-Via Napoli 25, Foggia, Italy.
J Chromatogr A. 2009 May 1;1216(18):3864-8. doi: 10.1016/j.chroma.2009.02.068. Epub 2009 Mar 3.
A rapid and efficient method using high performance liquid chromatography coupled with diode array detection and tandem mass spectrometry (HPLC-DAD-MS/MS) for fast screening large numbers of anthocyanins and anthocyanin dimers in different grape skin extracts, without further sample clean-up procedures, was developed. A good separation of most detected anthocyanins was achieved in a run time of 15 min. Identification of anthocyanin pigments required a combination of several information: UV-vis spectra, MS and MS/MS spectra, and elution pattern. Many compounds have been here detected for the first time and their structures tentatively elucidated.
开发了一种快速高效的方法，即使用高效液相色谱结合二极管阵列检测和串联质谱（HPLC-DAD-MS/MS），无需进一步的样品净化程序，即可快速筛选不同葡萄皮提取物中的大量花青素和花青素二聚体。在15分钟的运行时间内，大多数检测到的花青素实现了良好的分离。花青素色素的鉴定需要多种信息的结合：紫外可见光谱、质谱和串联质谱光谱以及洗脱模式。这里首次检测到许多化合物，并初步阐明了它们的结构。
