Flamini Riccardo, De Rosso Mirko, Smaniotto Anna, Panighel Annarita, Vedova Antonio Dalla, Seraglia Roberta, Traldi Pietro
CRA-VIT Laboratorio Chimico, Viale XXVIII aprile 26, 31015 Conegliano (TV), Italy.
Rapid Commun Mass Spectrom. 2009 Sep;23(18):2891-6. doi: 10.1002/rcm.4199.
In a previous work, direct-infusion electrospray ionization ion trap tandem mass spectrometry (ESI-IT-MS/MS) was applied to the study of anthocyanins in extracts from the skins of Clinton grapes, a non-Vitis vinifera red grape variety qualitatively and quantitatively rich in anthocyanins. A good characterization of anthocyaninins was obtained, but it was impossible to differentiate some compounds with the same nominal mass but with different elemental composition. In this work, the capabilities of quadrupole time-of-flight mass spectrometry (QTOF-MS) coupled with Chip-liquid chromatography (LC-Chip) were applied to the study of Clinton anthocyanins and this method provided the complete sample anthocyanin fingerprint in less than 5 min. Multi-stage mass spectrometry (MS(n); n >2) was not necessary to identify isobaric compounds, nor were deuterium-exchange experiments necessary to distinguish between compounds containing the same aglycone. The fast separation bypasses the problem of petunidin-3-O-(6-O-acetyl)monoglucoside and delphinidin-3,5-O-diglucoside quantification, present in the direct-infusion ESI-ITMS approach, due to overlapping with matrix interferences.
在之前的一项研究中，直接进样电喷雾电离离子阱串联质谱法（ESI-IT-MS/MS）被用于研究克林顿葡萄（一种非欧亚种红葡萄品种）果皮提取物中的花色苷，该品种富含花色苷，在定性和定量方面都有研究价值。对花色苷进行了很好的表征，但无法区分一些具有相同标称质量但元素组成不同的化合物。在这项工作中，将四极杆飞行时间质谱法（QTOF-MS）与芯片液相色谱法（LC-Chip）联用，用于研究克林顿葡萄花色苷，该方法在不到5分钟的时间内提供了完整的样品花色苷指纹图谱。对于等压化合物的鉴定，无需多级质谱法（MS(n)；n>2），对于含有相同糖苷配基的化合物，也无需进行氘交换实验来区分。由于与基质干扰重叠，直接进样ESI-ITMS方法中存在的矮牵牛素-3-O-(6-O-乙酰基)单葡萄糖苷和飞燕草素-3,5-O-二葡萄糖苷定量问题，在快速分离中得以避免。
