Liles M R, Newsome D A, Oliver P D
Sensory and Electrophysiology Research Unit, Touro Infirmary, New Orleans, LA 70115.
Arch Ophthalmol. 1991 Sep;109(9):1285-8. doi: 10.1001/archopht.1991.01080090111033.
The antioxidant enzymes catalase and superoxide dismutase have integral roles in controlling reactive oxygen radicals that can harm cells. In the present study, we quantitated catalase activity in retinal pigment epithelium, retina, iris, and vitreous from human donors. To our knowledge, our results represent the first quantitation of catalase activity in human retinal pigment epithelium and show six-fold greater catalase activity in retinal pigment epithelium than in other ocular tissues analyzed (P less than .0001). To investigate whether aging or macular degeneration affects retinal pigment epithelium catalase or superoxide dismutase activities, we measured enzyme levels in retinal pigment epithelium from donors 50 to 90 years of age with and without evidence of macular degeneration. Superoxide dismutase activity showed no significant correlations with aging or macular degeneration, while catalase activity decreased with age (P less than .02) and macular degeneration (P less than .05) in both macular and peripheral retinal pigment epithelium.
抗氧化酶过氧化氢酶和超氧化物歧化酶在控制可能损害细胞的活性氧自由基方面发挥着不可或缺的作用。在本研究中，我们对来自人类供体的视网膜色素上皮、视网膜、虹膜和玻璃体中的过氧化氢酶活性进行了定量分析。据我们所知，我们的结果代表了对人类视网膜色素上皮中过氧化氢酶活性的首次定量分析，结果显示视网膜色素上皮中的过氧化氢酶活性比所分析的其他眼组织高六倍（P小于0.0001）。为了研究衰老或黄斑变性是否会影响视网膜色素上皮过氧化氢酶或超氧化物歧化酶的活性，我们测量了50至90岁有或没有黄斑变性证据的供体视网膜色素上皮中的酶水平。超氧化物歧化酶活性与衰老或黄斑变性无显著相关性，而过氧化氢酶活性在黄斑和周边视网膜色素上皮中均随年龄增长（P小于0.02）和黄斑变性（P小于0.05）而降低。
