Kaledin V I, Llnitskaya S I, Nikolin V P, Popova N A, Smagin D A, Kudryavtseva N N
Institute of Cytology and Genetics, Siberian Department of the Russian Academy of Sciences, Novosibirsk 630090, Russia.
Exp Oncol. 2009 Mar;31(1):62-4.
It has been shown previously that chronic social defeat stress produces development of strong anxiety and increases intensity of experimental metastasis in the losers in comparison with the winners and control mice. The question was: is it possible to decrease the number of metastases in the losers by chronic or acute diazepam treatment.
Sensory contact model was used for generating male mice with repeated experience of social victories or defeats in daily agonistic interactions. Tumor cells of Lewis Lung Carcinoma (LLC) were injected into the tail vein of animals after 10 days of agonistic interactions. Then mice were treated acutely or chronically (7 days) with diazepam (1 mg/kg, i. p). Number of metastases in the lung was calculated in 16 days after tumor cell transplantation.
Diazepam decreased the number of LLC metastases in anxious losers, whereas in the winners and control mice, without anxiety state, diazepam was ineffective.
Well-known anxiolytic diazepam may decrease intensity of metastasis in anxious mice.
先前的研究表明，与获胜小鼠和对照小鼠相比，慢性社会挫败应激会导致强烈焦虑的产生，并增加失败小鼠实验性转移的强度。问题在于：通过慢性或急性地西泮治疗能否减少失败小鼠的转移灶数量。
采用感官接触模型，使雄性小鼠在日常的争斗互动中反复经历社会胜利或失败。在争斗互动10天后，将Lewis肺癌（LLC）肿瘤细胞注入动物尾静脉。然后，小鼠接受急性或慢性（7天）地西泮（1mg/kg，腹腔注射）治疗。在肿瘤细胞移植16天后计算肺内转移灶数量。
地西泮减少了焦虑的失败小鼠的LLC转移灶数量，而在无焦虑状态的获胜小鼠和对照小鼠中，地西泮无效。
众所周知的抗焦虑药物地西泮可能会降低焦虑小鼠的转移强度。
