Sckell Axel, Leunig Michael
Department of Trauma and Reconstructive Surgery, Charité University Medical Center, Berlin, Germany.
Methods Mol Biol. 2009;467:305-17. doi: 10.1007/978-1-59745-241-0_19.
Intravital microscopy represents an internationally accepted and sophisticated experimental method to study angiogenesis, microcirculation, and many other parameters in a wide variety of neoplastic and nonneoplastic tissues. Since 1924, when the first transparent chamber model in animals was introduced, many other chamber models have been described in the literature for studying angiogenesis and microcirculation. Because angiogenesis is an active and dynamic process, one of the major strengths of chamber models is the possibility of monitoring angiogenesis in vivo continuously for up to several weeks with high spatial and temporal resolution. In addition, after the termination of experiments, tissue samples can be excised easily and further examined by various in vitro methods, such as histology, immunohistochemistry, and molecular biology. This chapter describes the protocol for the surgical preparation of a dorsal skinfold chamber in mice as well as the method to implant tumors in this chamber for further investigations of angiogenesis and other microcirculatory parameters. However, the application of the dorsal skinfold chamber model is not limited to the investigation of neoplastic tissues. To this end, the investigation of angiogenesis and other microcirculatory parameters of nonneoplastic tissues such as tendons, osteochondral grafts, or pancreatic islets have been objects of interest.
活体显微镜检查是一种国际公认的、用于研究多种肿瘤组织和非肿瘤组织中血管生成、微循环及许多其他参数的精密实验方法。自1924年引入首个动物透明室模型以来，文献中已描述了许多其他用于研究血管生成和微循环的室模型。由于血管生成是一个活跃且动态的过程，室模型的主要优势之一是能够以高空间和时间分辨率在体内连续监测血管生成长达数周。此外，实验结束后，组织样本可轻松切除，并通过各种体外方法进一步检查，如组织学、免疫组织化学和分子生物学。本章描述了小鼠背部皮褶室的手术制备方案以及在该室中植入肿瘤以进一步研究血管生成和其他微循环参数的方法。然而，背部皮褶室模型的应用并不局限于肿瘤组织的研究。为此，对肌腱、骨软骨移植物或胰岛等非肿瘤组织的血管生成和其他微循环参数的研究也备受关注。
