Ushiyama Akira, Yamada Shigeyuki, Ohkubo Chiyoji
Department of Environmental Health, National Institute of Public Health, Tokyo, Japan.
Microvasc Res. 2004 Sep;68(2):147-52. doi: 10.1016/j.mvr.2004.05.004.
The dorsal skinfold chamber (DSC) is a transparent devise useful for the long-term study of subcutaneous microcirculation. Commonly used chambers are made of metal, thus heavy and potentially stress including. Therefore, we developed a nonmetallic dorsal skinfold chamber and demonstrated that it performs as well as metal chambers. The essential structural parts of the nonmetallic chamber are made of polyacetal resin (Duracon), and its design was based on an existing titanium chamber. Both Duracon and titanium chambers were implanted onto an 8-week-old male BALB/c mice and microcirculatory parameters were compared. In subcutaneous venules, there were no significant differences between the chambers in terms of maximum blood velocity, blood flow rate, or the number of rolling or adhering leukocytes in vessels. In arterioles, main frequency of vasomotion showed no significant difference between the two types of chambers. This novel nonmetallic chamber frame was determined to be as practical as the metal frame. The nonmetallic chamber is about 40% lighter in weight than the metallic one, subjecting mice to less stress. Additionally, the chamber has greater applications in experimental use where metallic frames are unsuitable, such as those employing nonionizing radiation.
背部皮褶腔室（DSC）是一种用于皮下微循环长期研究的透明装置。常用的腔室由金属制成，因此较重且可能产生应力。因此，我们开发了一种非金属背部皮褶腔室，并证明其性能与金属腔室相当。非金属腔室的基本结构部件由聚缩醛树脂（Duracon）制成，其设计基于现有的钛腔室。将Duracon腔室和钛腔室都植入8周龄雄性BALB/c小鼠体内，并比较微循环参数。在皮下小静脉中，两种腔室在最大血流速度、血流量或血管中滚动或黏附白细胞的数量方面没有显著差异。在小动脉中，两种类型腔室的血管运动主要频率没有显著差异。这种新型非金属腔室框架被确定与金属框架一样实用。非金属腔室的重量比金属腔室轻约40%，使小鼠承受的压力更小。此外，该腔室在金属框架不适用的实验用途中有更大的应用，例如那些采用非电离辐射的实验。
