Kallos J
Nature. 1977 Feb 24;265(5596):705-10. doi: 10.1038/265705a0.
The nature of the cyclic AMP-receptor-nucleus interactions was examined by a novel combination of two photoreactions. A photosensitive derivative of cyclic AMP, N6-butyryl cyclic AMP, was covalently attached to its cytoplasmic receptod by photo-affinity labelling and this receptor complex was photo-crosslinked by the DNA in the rat liver nuclei. The photolytic reactions seemed to be specific since stable links were formed only when substantial noncovalent binding occured.
通过两种光反应的新型组合研究了环磷酸腺苷-受体-细胞核相互作用的性质。环磷酸腺苷的一种光敏衍生物,N6-丁酰基环磷酸腺苷,通过光亲和标记共价连接到其细胞质受体上,并且这种受体复合物通过大鼠肝细胞核中的DNA进行光交联。光解反应似乎具有特异性,因为只有当发生大量非共价结合时才会形成稳定的连接。