Cho-Chung Y S, Archibald D, Clair T
Science. 1979 Sep 28;205(4413):1390-2. doi: 10.1126/science.224463.
Adenosine 3',5'-monophosphate (cyclic AMP) receptor protein of 56,000 daltons increases markedly in mammary tumors induced by 7,12-dimethylbenz[a]anthracene (DMBA) after incubation of tumor slices with cyclic AMP, benzamide, and arginine. Incubation of cytosol from these tumor slices with nuclei from unincubated tumors results in nuclear uptake of the 56,000-dalton cyclic AMP receptor and in phosphorylation of the 76,000-dalton nuclear protein. Binding of the 56,000-dalton receptor and phosphorylation of the 76,000-dalton protein also occur in DMBA tumor nuclei when protein kinase type II of bovine heart is used. The results suggest that cyclic AMP receptor is involved in the nuclear events of a hormone-dependent mammary tumor.
在将肿瘤切片与环磷酸腺苷、苯甲酰胺和精氨酸一起孵育后，由7,12-二甲基苯并[a]蒽（DMBA）诱导的乳腺肿瘤中，56,000道尔顿的环磷酸腺苷受体蛋白显著增加。将这些肿瘤切片的胞质溶胶与未孵育肿瘤的细胞核一起孵育，会导致56,000道尔顿的环磷酸腺苷受体被细胞核摄取，并使76,000道尔顿的核蛋白发生磷酸化。当使用牛心II型蛋白激酶时，DMBA肿瘤细胞核中也会发生56,道尔顿受体的结合以及76,000道尔顿蛋白的磷酸化。结果表明，环磷酸腺苷受体参与了激素依赖性乳腺肿瘤的核内事件。
