Messaros Bridget M, Rossano Mary G, Liu Guanghui, Diamond Michael P, Friderici Karen, Nummy-Jernigan Katherine, Daly Douglas, Puscheck Elizabeth, Paneth Nigel, Wirth Julia J
Department of Epidemiology, Michigan State University, A632 West Fee Hall, East Lansing, MI 48823, USA.
Environ Res. 2009 May;109(4):457-64. doi: 10.1016/j.envres.2009.02.009. Epub 2009 Mar 20.
Effects of ambient exposure to DDT and its metabolites (DDE-DDT) on human sperm parameters and the role of genetic polymorphisms in modifying the association were investigated.
Demographics, medical history data, blood and semen samples were obtained from the first 336 male partners of couples presenting to 2 infertility clinics. Serum was analyzed for organochlorines (OC) and DNA for polymorphisms in GSTM1, GSTT1, GSTP1 and CYP1A1. Men with each sperm parameter considered low by WHO criteria (concentration <20million/mL, motility <50%, morphology <4%) were compared to men with all normal sperm parameters in logistic regression models, controlling for sum of other OC pesticides.
High DDE-DDT level was associated with significantly increased odds for all 3 low sperm parameters. The risk of low motility with high DDE-DDT exposure was increased in men with the GSTT1 null genotype compared to those with GSTT1 intact (odds ratio (OR)=4.19, 95% confidence interval (CI) 1.05-16.78 and OR=3.57, 1.43-8.93, respectively). Risk for low morphology in men with high DDE-DDT and one or both CYP1A1*2A alleles was lower compared to men with the common CYP1A1 alleles (OR=2.18, 0.78-6.07 vs. OR=3.45, 1.32-9.03, respectively). Similar results were obtained for men with low DDE-DDT exposure. Effects of high DDE-DDT on low sperm concentration (OR=2.53, 1.0-6.31) was unaffected by the presence of the polymorphisms.
High DDE-DDT exposure adversely affected all 3 sperm parameters and its effects were exacerbated by the GSTT1 null polymorphism and by the CYP1A1 common alleles.
研究环境暴露于滴滴涕（DDT）及其代谢产物（DDE-DDT）对人类精子参数的影响，以及基因多态性在改变这种关联中的作用。
从前往两家不孕不育诊所就诊的夫妇的首批336名男性伴侣处获取人口统计学、病史数据、血液和精液样本。分析血清中的有机氯（OC），并分析GSTM1、GSTT1、GSTP1和CYP1A1基因的多态性。在逻辑回归模型中，将根据世界卫生组织标准每种精子参数被视为低水平的男性（浓度<2000万/mL、活力<50%、形态<4%）与所有精子参数均正常的男性进行比较，并控制其他OC农药的总量。
高DDE-DDT水平与所有3种低精子参数的显著增加的几率相关。与GSTT1完整的男性相比，GSTT1基因缺失型的男性在高DDE-DDT暴露下出现低活力的风险增加（优势比（OR）分别为4.19，95%置信区间（CI）1.05-16.78和OR=3.57，1.43-8.93）。与具有常见CYP1A1等位基因的男性相比，高DDE-DDT且携带一个或两个CYP1A1*2A等位基因的男性出现低形态的风险较低（OR分别为2.18，0.78-6.07和OR=3.45，1.32-9.03）。低DDE-DDT暴露的男性也得到了类似结果。高DDE-DDT对低精子浓度的影响（OR=2.53，1.0-6.31）不受多态性存在的影响。
高DDE-DDT暴露对所有3种精子参数均产生不利影响，并且GSTT1基因缺失多态性和CYP1A1常见等位基因会加剧其影响。
