γδ T细胞对Nod2激动剂胞壁酰二肽的独特反应。
The distinct response of gammadelta T cells to the Nod2 agonist muramyl dipeptide.
作者信息
Kerns Hannah M M, Jutila Mark A, Hedges Jodi F
机构信息
Veterinary Molecular Biology, Montana State University, Bozeman, MT 59718, USA.
出版信息
Cell Immunol. 2009;257(1-2):38-43. doi: 10.1016/j.cellimm.2009.02.004.
Abstract
Purified gammadelta T cells are primed directly in response to pathogen associated molecular patterns (PAMPs) to better respond to secondary signals and increase expression of chemokine and activation-related genes. Transcripts encoding the innate receptor Nod2 were detected in bovine and human gammadelta T cells. Nod2 is the intracellular receptor for muramyl dipeptide (MDP), functions in regulating innate activities, and was thought to be expressed primarily in APCs. The response of gammadelta T cells to MDP was analyzed by microarray, Q-PCR, proteome array and functional priming assays. MDP had a consistent priming effect on gammadelta T cells, characterized by changes in transcripts and enhanced proliferation response to secondary signaling. Knockdown experiments implicated Nod2 as the receptor for MDP in gammadelta T cell-enriched bovine PBLs. The results indicate priming of gammadelta T cells by MDP, and offer definitive evidence of the expression of functional Nod2 in gammadelta T cells.
摘要
纯化的γδ T细胞可直接被激活，以响应病原体相关分子模式（PAMP），从而更好地响应二级信号，并增加趋化因子和激活相关基因的表达。在牛和人的γδ T细胞中检测到了编码天然受体Nod2的转录本。Nod2是胞壁酰二肽（MDP）的细胞内受体，在调节天然免疫活动中发挥作用，曾被认为主要在抗原呈递细胞（APC）中表达。通过微阵列、定量聚合酶链反应（Q-PCR）、蛋白质组阵列和功能激活测定法分析了γδ T细胞对MDP的反应。MDP对γδ T细胞具有一致的激活作用，其特征在于转录本的变化以及对二级信号的增殖反应增强。敲低实验表明，在富含γδ T细胞的牛外周血淋巴细胞（PBL）中，Nod2是MDP的受体。结果表明MDP可激活γδ T细胞，并为功能性Nod2在γδ T细胞中的表达提供了确凿证据。
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