Janiec W, Piekarska T, Szczypior M, Misterkiewicz E
Pol J Pharmacol Pharm. 1977 Mar-Apr;29(2):93-9.
The content of cyclic AMP (cAMP) in the slices of rat striatum and cerebral cortex was tested after incubation with various concentrations (10(-6)--10(4) M) of dopamine (DA), apomorphine (APM), amantadine (AMD), and dimethylaminoadamatane (DMAD), and incubation with DA, AMD and DMAD in combination with KCl (43 mM) or haloperidol (HP) (10(-5) M). APM, AMD, and DMAD act synergistically with DA, elevate the c-AMP content in the striatum less than DA dose and do not affect the level c-AMP in the slices of cerebral cortex. KCl potentiated the action of DA, but inhibited that of APM, AMD, and DMAD. HP was most effective in blocking the action of DA, and blocked more weakly the action of APM AMD, DMAD, and the action of DA, APM, AMD, and DMAD in the presence of high concentration of KCl. The results indicate that APM, AMD, and DMAD, are less specific stimulants of central dopaminergic structures than DA, and that the increase of c-AMP content in the slices of rat striatum caused by APM, AMD, and DMAD is due to depolarizing action of these compounds.
将大鼠纹状体和大脑皮层切片与不同浓度（10⁻⁶ - 10⁴ M）的多巴胺（DA）、阿扑吗啡（APM）、金刚烷胺（AMD）和二甲氨基金刚烷（DMAD）孵育后，以及与DA、AMD和DMAD联合KCl（43 mM）或氟哌啶醇（HP）（10⁻⁵ M）孵育后，检测其环磷酸腺苷（cAMP）含量。APM、AMD和DMAD与DA协同作用，使纹状体中的cAMP含量升高，但升高幅度小于DA剂量，且不影响大脑皮层切片中的cAMP水平。KCl增强了DA的作用，但抑制了APM、AMD和DMAD的作用。HP在阻断DA的作用方面最有效，在高浓度KCl存在的情况下，对APM、AMD、DMAD以及DA、APM、AMD和DMAD联合作用的阻断作用较弱。结果表明，与DA相比，APM、AMD和DMAD对中枢多巴胺能结构的刺激作用特异性较低，并且APM、AMD和DMAD引起大鼠纹状体切片中cAMP含量增加是由于这些化合物的去极化作用。
