Janiec W, Piekarska T, Pałetko Z, Pytlik M
Pol J Pharmacol Pharm. 1978 Jul-Aug;30(4):529-35.
We tested the effect of dopamine (DA), apomorphine (APM), amantadine (AMD), dimethylaminoadamantane (DMAD) or haloperidol (HP) on the in vitro formation of c-AMP from ATP labelled intracellularly with exogenous 14C-adenine or from endogenous ATP in the slices of rat striatum. DA stimulated the synthesis of c-AMP from endogenous and 14C-adenine labelled ATP. Nonspecific stimulants of the dopaminergic system, APM, AMD and DMAD, stimulated the c-AMP synthesis much weaker than DA did. After application of APM, ADM or DMAD, the utilization of ATP formed from 14C/U/adenine for synthesis of c-AMP was lower than after DA. The effects of combined treatment with DA together with APM, AMD or DMAD was additive. HP, 1 X 10(-3) M did not change the amount of radioactive and total c-AMP, but inhibited the stimulatory action of DA and, to a lesser degree, of APM, AMD and DMAD. The synthesis of c-AMP from endogenous ATP stimulated with APM, AMD and DMAD in striate body slices was inhibited by HP more strongly than the synthesis of c-AMP from ATP labelled with exogenous 14C-adenine. The results indicate that ATP synthesized from intracellular adenyl nucleotides is more effectively utilized for the synthesis of c-AMP stimulated by APM, AMD or DMAD in striate body slices, than ATP labelled with 14C-adenine.
我们测试了多巴胺（DA）、阿扑吗啡（APM）、金刚烷胺（AMD）、二甲氨基金刚烷（DMAD）或氟哌啶醇（HP）对大鼠纹状体切片中，由外源性14C-腺嘌呤细胞内标记的ATP或内源性ATP体外形成环磷酸腺苷（c-AMP）的影响。DA刺激了内源性和14C-腺嘌呤标记的ATP合成c-AMP。多巴胺能系统的非特异性兴奋剂APM、AMD和DMAD刺激c-AMP合成的作用比DA弱得多。应用APM、ADM或DMAD后，由14C/U/腺嘌呤形成的ATP用于合成c-AMP的利用率低于DA作用后。DA与APM、AMD或DMAD联合处理的效果是相加的。1×10-3M的HP不改变放射性c-AMP和总c-AMP的量，但抑制DA的刺激作用，并在较小程度上抑制APM、AMD和DMAD的刺激作用。HP对纹状体切片中由APM、AMD和DMAD刺激的内源性ATP合成c-AMP的抑制作用，比对由外源性14C-腺嘌呤标记的ATP合成c-AMP的抑制作用更强。结果表明，在纹状体切片中，由细胞内腺苷酸合成的ATP比用14C-腺嘌呤标记的ATP更有效地用于APM、AMD或DMAD刺激的c-AMP合成。
