Rukhadze Marina, Sebiskveradze Maya, Makharadze Theona, Kutkhashvili Maya
Faculty of Exact and Natural Sciences, Ivane Javakhishvili Tbilisi State University, 0128 Tbilisi, Georgia.
Biomed Chromatogr. 2009 Jul;23(7):700-6. doi: 10.1002/bmc.1166.
The chromatographic behavior of model compounds of biomedical significance (organic acids, amino acids, drugs) was investigated using mobile phases modified with tyloxapol. The influence of factors such as concentration of tyloxapol, content of organic modifier and pH of mobile phase on the retention factor of solutes was studied. The results were compared with the data obtained by elution with mobile phases containing Triton X-100 additives, since units of Triton X-100 are repeated in the structure of tyloxapol. Divergence in chromatographic behavior of model compounds was explained by the difference in physico-chemical characteristics (microviscosity, polarity, critical micelle concentration, shape of micelles, etc.) of tyloxapol and Triton X-100 micelles.
使用经泰洛沙泊改性的流动相研究了具有生物医学意义的模型化合物（有机酸、氨基酸、药物）的色谱行为。研究了泰洛沙泊浓度、有机改性剂含量和流动相pH值等因素对溶质保留因子的影响。将结果与含Triton X-100添加剂的流动相洗脱得到的数据进行了比较，因为泰洛沙泊结构中重复了Triton X-100单元。模型化合物色谱行为的差异是由泰洛沙泊和Triton X-100胶束的物理化学特性（微粘度、极性、临界胶束浓度、胶束形状等）的差异来解释的。
