通过对单个细胞的细胞表面和DNA进行同步荧光分析，对混合群体中的转化细胞进行定量测定。
Quantitative determination of transformed cells in a mixed population by stimultaneous fluorescence analysis of cell surface and DNA an individual cells.
作者信息
Hawkes S P, Bartholomew J C
出版信息
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1626-30. doi: 10.1073/pnas.74.4.1626.
Abstract
Cell-surface labeling with fluorescamine indicates that the fluorescence of Balb 3T3 A31 cells in considerably decreased after both viral and chemical transformation. This phenomenon, coupled with the technique of flow microfluorometry, enabled nontransformed and transformed cells to be distinguished. A second fluroescent probe, propidium iodide, which intercalates into DNA, was used in combination with fluorescamine in order to obtain a ratio of cell-surface labeling to DNA content. This manipulation allowed enhanced resolution of two populations and the detection of small numbers of transformants in a predominantly normal population.
摘要
用荧光胺进行细胞表面标记表明，在病毒转化和化学转化后，Balb 3T3 A31细胞的荧光显著降低。这一现象，再加上流动微荧光测定技术，使得未转化细胞和转化细胞得以区分。第二种荧光探针碘化丙啶可插入DNA中，它与荧光胺联合使用，以获得细胞表面标记与DNA含量的比率。这种操作提高了两个群体的分辨率，并能在主要为正常细胞的群体中检测到少量的转化细胞。
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2
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Proc Soc Exp Biol Med. 1967 Jun;125(2):367-70. doi: 10.3181/00379727-125-32093.
3
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Science. 1967 Jun 9;156(3780):1364-5. doi: 10.1126/science.156.3780.1364.
4
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8
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