Wyroba E, Bottiroli G, Giordano P
Histochemistry. 1983;77(2):171-5. doi: 10.1007/BF00506559.
Cytofluorimetric analysis of cycloheptaamylose-dansyl chloride (CDC) labelled Paramecium indicates that after mild trypsin removal of surface components the localization of CDC on the outer surface of living cells was not modified by the treatment. After such treatment the intensity of fluorescence emission was found about 3-fold higher in treated single cell than in the untreated one. These findings indicate that CDC labelling can be used to follow alteration occurred on the membrane of the living cell prior to labelling.
环庚糖-丹磺酰氯(CDC)标记草履虫的细胞荧光分析表明,在用温和的胰蛋白酶去除表面成分后,活细胞外表面上CDC的定位并未因该处理而改变。经过这种处理后,发现处理过的单细胞中的荧光发射强度比未处理的单细胞高约3倍。这些发现表明,CDC标记可用于追踪标记前活细胞膜上发生的变化。