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去除表面成分后,经CDC标记的草履虫的膜荧光增强。

Enhanced membrane fluorescence of CDC-labelled paramecium subsequent to removal of surface components.

作者信息

Wyroba E, Bottiroli G, Giordano P

出版信息

Histochemistry. 1983;77(2):171-5. doi: 10.1007/BF00506559.

DOI:10.1007/BF00506559
PMID:6841142
Abstract

Cytofluorimetric analysis of cycloheptaamylose-dansyl chloride (CDC) labelled Paramecium indicates that after mild trypsin removal of surface components the localization of CDC on the outer surface of living cells was not modified by the treatment. After such treatment the intensity of fluorescence emission was found about 3-fold higher in treated single cell than in the untreated one. These findings indicate that CDC labelling can be used to follow alteration occurred on the membrane of the living cell prior to labelling.

摘要

环庚糖-丹磺酰氯(CDC)标记草履虫的细胞荧光分析表明,在用温和的胰蛋白酶去除表面成分后,活细胞外表面上CDC的定位并未因该处理而改变。经过这种处理后,发现处理过的单细胞中的荧光发射强度比未处理的单细胞高约3倍。这些发现表明,CDC标记可用于追踪标记前活细胞膜上发生的变化。

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Histochemistry. 1983;77(2):171-5. doi: 10.1007/BF00506559.
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Basic Appl Histochem. 1985;29(2):121-33.
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本文引用的文献

1
A comparison of the tryptic peptides obtained from immobilization antigens of Paramecium aurelia.对从双小核草履虫固定化抗原中获得的胰蛋白酶肽段的比较。
Proc Natl Acad Sci U S A. 1962 May 15;48(5):867-74. doi: 10.1073/pnas.48.5.867.
2
Release of Paramecium immobilization antigen to the non-nutrient medium.草履虫固定抗原释放至无营养培养基中。
Cell Biol Int Rep. 1980 Jan;4(1):1-10. doi: 10.1016/0309-1651(80)90002-8.
3
Cycloheptaamylose-dansyl chloride complex as a fluorescent label of surface membranes in living ciliates.
Histochemistry. 1981 Dec;73(3):459-67. doi: 10.1007/BF00495660.
4
Concerning the tertiary structure of the soluble surface proteins of Paramecium.关于草履虫可溶性表面蛋白的三级结构。
Biochemistry. 1969 Nov;8(11):4637-44. doi: 10.1021/bi00839a061.
5
Organization of glycolipids and glycoproteins in surface membranes: dependency on cell cycle and on transformation.表面膜中糖脂和糖蛋白的组织:对细胞周期和转化的依赖性。
Biochem Biophys Res Commun. 1974 Jul 10;59(1):283-91. doi: 10.1016/s0006-291x(74)80204-4.
6
Enhancement of the fluorescence intensity of dansyl amino acids in aqueous media and its application to assay of amino acids.
Biochem Biophys Res Commun. 1973 Apr 2;51(3):666-71. doi: 10.1016/0006-291x(73)91366-1.
7
Transmembrane control of the receptors on normal and tumor cells. I. Cytoplasmic influence over surface components.正常细胞和肿瘤细胞上受体的跨膜调控。I. 细胞质对表面成分的影响。
Biochim Biophys Acta. 1976 Apr 13;457(1):57-108. doi: 10.1016/0304-4157(76)90014-9.
8
The use of fluorescamine as a probe for labeling the outer surface of the plasma membrane.
Biochem Biophys Res Commun. 1976 Feb 23;68(4):1226-33. doi: 10.1016/0006-291x(76)90328-4.
9
Studies on the surface coat of Paramecium aurelia. II. Relationship to the immobilization antigen.双小核草履虫表膜的研究。II. 与固定抗原的关系。
Cell Tissue Res. 1977 Jul 11;181(2):245-53. doi: 10.1007/BF00219984.
10
Cell multiplication following partial enzymatic removal of surface coat.部分酶解去除表面被膜后的细胞增殖
Cytobiologie. 1978 Aug;17(2):412-20.