Sandell Mari, Laaksonen Oskar, Järvinen Riikka, Rostiala Nina, Pohjanheimo Terhi, Tiitinen Katja, Kallio Heikki
Department of Biochemistry and Food Chemistry, University of Turku, Turku 20014, Finland.
J Agric Food Chem. 2009 May 13;57(9):3718-28. doi: 10.1021/jf803884y.
Fractionation of black currant ( Ribes nigrum ) by juice pressing, four ethanol extractions, ethanol evaporation, and supercritical fluid extraction was studied. Phenolic compounds, sugars, and acids of the fractions were analyzed by high-performance liquid chromatography and gas chromatography. Sensory properties of the fractions were studied using generic descriptive analysis. Most of the sugars and acids were located in the juice, whereas the majority of the phenolic compounds were in the press residue. Ethanol extracted nearly all of the phenolic compounds from the press residue, leaving only fibers and seeds. The juice was dominant in most of the sensory attributes, whereas the extracts were perceived as most astringent. Three flavonol glycosides [kaempferol-3-O-(6''-malonyl)glucoside, myricetin-3-O-galactoside, and an unknown kaempferol glycoside] were discovered to be the compounds especially contributing to astringency. Ethanol extraction appeared to be an efficient and simple way to isolate phenolic compounds from black currant juice press residue.
研究了通过榨汁、四次乙醇萃取、乙醇蒸发和超临界流体萃取对黑加仑(黑醋栗)进行分级分离。通过高效液相色谱法和气相色谱法分析了各馏分中的酚类化合物、糖类和酸类。使用通用描述性分析研究了各馏分的感官特性。大部分糖类和酸类存在于果汁中,而大多数酚类化合物存在于压榨残渣中。乙醇从压榨残渣中萃取出了几乎所有的酚类化合物,仅留下纤维和种子。果汁在大多数感官属性方面占主导地位,而提取物被认为最具涩味。发现三种黄酮醇糖苷[山奈酚-3-O-(6''-丙二酰)葡萄糖苷、杨梅素-3-O-半乳糖苷和一种未知的山奈酚糖苷]是特别导致涩味的化合物。乙醇萃取似乎是从黑加仑汁压榨残渣中分离酚类化合物的一种高效且简单的方法。