State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China.
College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China.
Molecules. 2018 Nov 30;23(12):3148. doi: 10.3390/molecules23123148.
Differences in the content of nine phenols and the antioxidant capacity of Maxim (RSM) fruits at different stages of maturity were investigated, and the extraction process of polyphenols from RSM was also optimized using Box-Behnken design method. Results showed that the content of the nine phenols varied considerably at different ripening stages; catechin, chlorogenic acid, coumaric acid, and ferulic acid were abundant in immature fruits but decreased with fruit ripening, whereas the levels of rosemary acid and querctin acid were low in immature fruits and increased with time, reaching the highest value after the fruit was completely mature. The phenols extracted from RSM fruits possessed good antioxidant activities for effective and rapid scavenging of DPPH and ABTS free radicals, as well as intracellular ROS. Analysis of the phenols content at different maturity stages indicated that the unripe fruits had significantly higher polyphenols content than mature fruits. Consequently, unripe fruits possessed higher antioxidant activities. According to the overall results of the extraction process optimization, the selected optimal conditions for extracting polyphenols from RSM were as follows: extraction time, 95 min; solvent concentration, 60%; ratio of sample to solvent, 1:25.
研究了不同成熟阶段的 Maxim（RSM）果实中九种酚类物质的含量和抗氧化能力差异，并采用 Box-Behnken 设计方法对 RSM 中多酚的提取工艺进行了优化。结果表明，九种酚类物质在不同成熟阶段的含量差异很大；在未成熟果实中，儿茶素、绿原酸、香豆酸和阿魏酸含量丰富，但随着果实成熟而减少，而迷迭香酸和槲皮素酸在未成熟果实中含量较低，随着时间的推移而增加，在果实完全成熟后达到最高值。从 RSM 果实中提取的酚类物质具有良好的抗氧化活性，能有效快速清除 DPPH 和 ABTS 自由基以及细胞内 ROS。不同成熟阶段酚类物质含量分析表明，未成熟果实的多酚含量明显高于成熟果实。因此，未成熟果实具有更高的抗氧化活性。根据提取工艺优化的综合结果，从 RSM 中提取多酚的最佳条件为：提取时间 95min，溶剂浓度 60%，料液比 1:25。
