Danchenko Maksym, Skultety Ludovit, Rashydov Namik M, Berezhna Valentyna V, Mátel L'ubomír, Salaj Terézia, Pret'ová Anna, Hajduch Martin
Department of Reproduction and Developmental Biology, Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia.
J Proteome Res. 2009 Jun;8(6):2915-22. doi: 10.1021/pr900034u.
The explosion in one of the four reactors of the Chernobyl Nuclear Power Plant (CNPP, Chernobyl) caused the worst nuclear environmental disaster ever seen. Currently, 23 years after the accident, the soil in the close vicinity of CNPP is still significantly contaminated with long-living radioisotopes, such as (137)Cs. Despite this contamination, the plants growing in Chernobyl area were able to adapt to the radioactivity, and survive. The aim of this study was to investigate plant adaptation mechanisms toward permanently increased level of radiation using a quantitative high-throughput proteomics approach. Soybeans of a local variety (Soniachna) were sown in contaminated and control fields in the Chernobyl region. Mature seeds were harvested and the extracted proteins were subjected to two-dimensional gel electrophoresis (2-DE). In total, 9.2% of 698 quantified protein spots on 2-D gel were found to be differentially expressed with a p-value </= 0.05. All differentially expressed spots were excised from the 2-D gels and analyzed by tandem mass spectrometry. Identified differentially expressed proteins were categorized into six main metabolic classes. Most abundant functional classes were associated with protein destination and storage followed by disease and defense. On the basis of the identity of these proteins, a working model for plant adaptation toward radio-contaminated Chernobyl soil conditions was proposed. Our results suggest that adaptation toward heavy metal stress, protection against radiation damage, and mobilization of seed storage proteins are involved in plant adaptation mechanism to radioactivity in the Chernobyl region.
切尔诺贝利核电站（CNPP，切尔诺贝利）四个反应堆之一发生的爆炸导致了有史以来最严重的核环境灾难。目前，事故发生23年后，CNPP附近的土壤仍被长寿命放射性同位素如（137）Cs严重污染。尽管存在这种污染，切尔诺贝利地区生长的植物仍能够适应放射性并存活下来。本研究的目的是使用定量高通量蛋白质组学方法研究植物对辐射水平持续升高的适应机制。将当地品种（Soniachna）的大豆播种在切尔诺贝利地区受污染和对照田地中。收获成熟种子，提取的蛋白质进行二维凝胶电泳（2-DE）。在2-D凝胶上总共698个定量蛋白质点中，发现9.2%的点差异表达，p值≤0.05。从2-D凝胶上切下所有差异表达的点，通过串联质谱分析。鉴定出的差异表达蛋白质被分为六个主要代谢类别。最丰富的功能类别与蛋白质目的地和储存相关，其次是疾病和防御。基于这些蛋白质的特性，提出了植物对受放射性污染的切尔诺贝利土壤条件适应的工作模型。我们的结果表明，对重金属胁迫的适应、对辐射损伤的保护以及种子储存蛋白的动员参与了切尔诺贝利地区植物对放射性的适应机制。
