Donetti Elena, Bedoni Marzia, Capone Paolo, Gualerzi Alice, Tartaglia Gianluca, Sforza Chiarella
Laboratory of Immunohistochemistry of Epithelia, Department of Human Morphology, Faculty of Medicine and Surgery, University of Milan, Via Mangiagalli 31, Milan, Italy.
Eur J Oral Sci. 2009 Apr;117(2):169-74. doi: 10.1111/j.1600-0722.2009.00614.x.
Mucositis is a frequent problem after irradiation of the oral mucosa. To study the early effects of irradiation on the desmosomal adhesion complex, explants of keratinized oral mucosa were exposed to a single dose of 2 Gy gamma irradiation. Biopsies were obtained from the upper dental arch of nine young healthy non-smoking women undergoing minor oral surgery. The biopsies were incubated in a Transwell culture system and, after irradiation, fixed in formalin 24 h later. Morphometric measurements of epithelial thickness revealed that it was unaffected by exposure to ionizing rays. Immunofluorescence analysis of desmosomal cadherin expression (desmoglein 1/desmoglein 3) demonstrated that the distribution of desmoglein 1 was not affected, whereas the expression of desmoglein 3 decreased in the suprabasal layers of irradiated samples. It is suggested that this has consequences for the mechanical integrity of the mucosa and promotes the development of radiation mucositis.
口腔黏膜炎是口腔黏膜照射后常见的问题。为研究照射对桥粒黏附复合体的早期影响，将角化口腔黏膜外植体暴露于2 Gy的单次γ射线照射。从9名接受小型口腔手术的年轻健康非吸烟女性的上牙弓获取活检组织。将活检组织在Transwell培养系统中培养，照射后24小时用福尔马林固定。上皮厚度的形态计量学测量显示，其不受电离射线暴露的影响。桥粒钙黏蛋白表达（桥粒芯糖蛋白1/桥粒芯糖蛋白3）的免疫荧光分析表明，桥粒芯糖蛋白1的分布未受影响，而照射样本的基底上层中桥粒芯糖蛋白3的表达降低。提示这对黏膜的机械完整性有影响，并促进放射性口腔黏膜炎的发展。
